Phage display : a practical approach / edited by Tim Clackson, Henry B. Lowman.

Format:

Book

Contributor:

Clackson, Tim, editor.

Lowman, Henry B., editor.

Series:

Practical approach series ; no. 266.

Oxford scholarship online.

Practical approach series ; no. 266

Oxford scholarship online

Language:

English

Subjects (All):

Viral proteins.

Bacteriophages.

Microbial biotechnology.

Physical Description:

1 online resource (357 p.)

Edition:

1st ed.

Place of Publication:

Oxford : Oxford University Press, 2023.

Language Note:

English

Summary:

Phage display has become established as a powerful protein engineering method for identifying polypeptides with novel properties, and altering the properties of existing ones. Although the technique is widely used in biological research and drug discovery, it remains technically challenging, and new applications and procedures continue to evolve. Phage Display - A Practical Approach is an up-to-date, comprehensive and integrated experimental guide to the technique, useful for novice and expert alike. The book aims to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analysing the results.

Contents:

Contents; Protocol list; List of abbreviations; List of contributors; 1 Introduction to phage biology and phage display; 2 Constructing phage display libraries by oligonucleotide-directed mutagenesis; 3 In vitro DNA recombination; 4 Phage selection strategies for improved affinity and specificity of proteins and peptides; 5 Rapid screening of phage displayed protein binding affinities by phage ELISA; 6 Identification of novel ligands for receptors using recombinant peptide libraries; 7 Substrate phage display

8 Protease-based selection of stably folded proteins and protein domains from phage display libraries9 Phage display of zinc fingers and other nucleic acid-binding motifs; 10 In vivo and ex vivo selections using phage-displayed libraries; 11 Screening phage libraries with sera; 12 Interaction cloning using cDNA libraries displayed on phage; 13 Phage antibody libraries; 14 Affinity maturation of phage antibodies; Appendix: Non-standard suppliers; Index;

Notes:

Previously issued in print: 2004.

Includes bibliographical references and index.

Description based on print version record.

ISBN:

1-383-05013-9

1-280-84529-5

0-19-151645-7

OCLC:

476259877