ENZYMES: Catalysis, Kinetics and Mechanisms / by N.S. Punekar.

Format:

Book

Author/Creator:

Punekar, N. S. (Narayan S.), author.

Contributor:

SpringerLink (Online service)

Series:

Biomedical and Life Sciences (Springer-11642)

Language:

English

Subjects (All):

Enzymes.

RNA-ligand interactions.

Biomedical engineering.

Microbiology.

Biochemistry.

Enzymology.

Protein-Ligand Interactions.

Biomedical Engineering/Biotechnology.

Applied Microbiology.

Protein Structure.

Local Subjects:

Enzymology.

Protein-Ligand Interactions.

Biomedical Engineering/Biotechnology.

Applied Microbiology.

Protein Structure.

Physical Description:

1 online resource (XXIII, 562 pages) : 263 illustrations, 76 illustrations in color

Edition:

First edition 2018.

Contained In:

Springer eBooks

Place of Publication:

Singapore : Springer Singapore : Imprint: Springer, 2018.

System Details:

text file PDF

Summary:

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Contents:

Part I: Enzyme catalysis - a perspective

Enzymes -Their place in Biology

Enzymes - Historical aspects

Exploiting enzymes - Technology

On enzyme nomenclature and classification

Hallmarks of an enzyme catalyst

Origins of enzyme catalytic power

Which enzyme uses what trick? Some remarks

Structure and catalysis: Conformational flexibility and protein motion

Part II: Enzyme kinetic practice and measurements

Chemical kinetics - Fundamentals

Concepts of equilibrium and steady-state

ES complex and pre-steady-state kinetics

Principles of enzyme assays

Good kinetic practices

Quantification of catalysis and measures of enzyme purity

Henri-Michaelis-Menten equation

More complex rate expressions

Enzyme kinetic data - Collection and analysis

Part III: Elucidation of Kinetic mechanisms

Approaches to kinetic mechanism - Overview

Analysis of initial velocity patterns

Enzyme inhibition analyses

Irreversible inhibitions

Reversible inhibitions

Alternate substrate (product) interactions

pH Studies with enzymes

Isotopes in enzymology

Isotope exchanges at equilibrium

Isotope effects in enzymology

From kinetic data to mechanism and back

Part IV: Chemical mechanisms and catalysis

Chemical reactivity and molecular interactions

Acid-base chemistry and catalysis

Nucleophilic catalysis and covalent reaction intermediates

Phosphoryl group chemistry and importance of ATP

Enzymatic oxidation-reduction reactions

Carboxylations and decarboxylations

Electrophilic catalysis and amino acid transformations

Integrating kinetic and chemical mechanisms - A synthesis

Part V: Frontiers in enzymology

Regulation of enzyme activity

In vitro versus in vivo - Concept and consequences

Future of enzymology - An appraisal

Closure - Whither Enzymology.

Other Format:

Printed edition:

ISBN:

978-981-13-0785-0

9789811307850

Access Restriction:

Restricted for use by site license.