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High-Throughput Metabolomics : Methods and Protocols / edited by Angelo D'Alessandro.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
D'Alessandro, Angelo, Ph. D., editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 1978.
Methods in Molecular Biology, 1064-3745 ; 1978
Language:
English
Subjects (All):
Metabolism.
Human genetics.
Lipids.
Bioinformatics.
Metabolomics.
Human Genetics.
Lipidology.
Computational Biology/Bioinformatics.
Local Subjects:
Metabolomics.
Human Genetics.
Lipidology.
Computational Biology/Bioinformatics.
Physical Description:
1 online resource (XV, 459 pages) : 83 illustrations, 63 illustrations in color.
Edition:
First edition 2019.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana, 2019.
System Details:
text file PDF
Summary:
This detailed volume focuses on recent technological, computational, and biostatistical advances in the field of high-throughput metabolomics. Chapters encompass methods, platforms, and analytical strategies for steady state measurements and metabolic flux analysis with stable isotope-labeled tracers, in biological matrices of clinical relevance and model organisms. Mass spectrometry-based or orthogonal methods are discussed, along with computational and statistical methods to address data sparsity in high-throughput metabolomics approaches. As a part of the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Metabolomics: Methods and Protocols provides tools that can bring about the next generation of clinical biochemistry in a cost-effective, rigorous fashion, exponentially advancing our capacity to investigate nature while hastening the advent of personalized medicine.
Contents:
Sample Preparation and Reporting Standards for Metabolomics of Adherent Mammalian Cells
High-Throughput Metabolomics: Isocratic and Gradient Mass Spectrometry-Based Methods
High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research
Traveling Wave Ion Mobility Mass Spectrometry: Metabolomics Applications
CE-MS for Metabolomics: A Comparison with Other Techniques
Overview of Lipid Mass Spectrometry and Lipidomics
LC-MS/MS-MRM-Based Targeted Metabolomics for Quantitative Analysis of Polyunsaturated Fatty Acids and Oxylipins
Untargeted and Semi-Targeted Lipid Analysis of Biological Samples Using Mass Spectrometry-Based Metabolomics
HPLC-MS/MS Methods for Diacylglycerol and Sphingolipid Molecular Species in Skeletal Muscle
Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry
Comprehensive LC-MS-Based Metabolite Fingerprinting Approach for Plant and Fungal-Derived Samples
Untargeted Metabolomics of Plant Leaf Tissues
Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling
Quantifying Intermediary Metabolism and Lipogenesis in Cultured Mammalian Cells Using Stable Isotope Tracing and Mass Spectrometry
Insights into Dynamic Network States Using Metabolomic Data
Analysis of Endothelial Fatty Acid Metabolism Using Tracer Metabolomics
Using Stable Isotope Tracers for Metabolic Pathway Analysis
Data Processing for GC-MS and LC-MS-Based Untargeted Metabolomics
El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics
Pre-Analytic Considerations for Mass Spectrometry-Based Untargeted Metabolomics Data
Temporal Metabolite, Ion, and Enzyme Activity Profiling Using Fluorescence Microscopy and Genetically-Encoded Biosensors
Microplate Assays for Spectrophotometric Measurement of Mitochondrial Enzyme Activity
Quantitative NMR-Based Metabolomics on Tissue Biomarkers and Its Translation into In Vivo Magnetic Resonance Spectroscopy
Metabolomic Applications in Radiation Biodosimetry
Metabolomics Analyses to Investigate the Role of Diet and Physical Training
Blood Biomarkers in Sports Medicine and Performance and the Future of Metabolomics
Personalized Metabolomics.
Other Format:
Printed edition:
ISBN:
978-1-4939-9236-2
9781493992362
Access Restriction:
Restricted for use by site license.

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