Metabolomics : Methods and Protocols / edited by Sanjoy K. Bhattacharya.

Format:

Book

Contributor:

Bhattacharya, Sanjoy K., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1996.

Methods in Molecular Biology, 1064-3745 ; 1996

Language:

English

Subjects (All):

Metabolism.

Mass spectrometry.

Metabolomics.

Mass Spectrometry.

Local Subjects:

Metabolomics.

Mass Spectrometry.

Physical Description:

1 online resource (XV, 335 pages) : 82 illustrations, 53 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

The volume brings together some of the best experts in the field of modern metabolomics to discuss various techniques used today to study specific metabolite classes, and metabolomics in bacterial systems and mammalian systems. The chapters in this book cover topics such as Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial siderophores analysis by mass spectrometry; the metabolomic study of tissues in Parkinson's Disease; and NMR analysis in livestock metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, tips on troubleshooting and avoiding pitfalls. Cutting-edge and thorough, Metabolomics: Methods and Protocols is an essential resource for any researcher interested in this exciting and evolving field. .

Contents:

Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer

Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis

Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals

Quantitative Metabolomics using Isotope Residue Outlier Analysis (IROA®) with Internal Standards

Analyses of Cholesterol Metabolites of Optic Nerve using GC-MS Methods

Analyses of Cholesterol and Derivatives in Ocular Tissues using LC-MS/MS Methods

Measurement of Pyridine Nucleotides in Biological Samples using LC-MS/MS

Handling and Analysis of 5-Formyl-, 5,10-Methenyl-, 10-Formyl-, 5-Formimno-, and 5,10-Methylenetetrahydrofolates

Protein-Lipid Complex Separation Utilizing a Capillary Electrophoresis System

Immature and Mature Collagen Crosslinks Quantification using High Performance Liquid Chromatography and High Resolution Mass Spectrometry in OrbitrapTM

Relative Quantitation of Neurotoxic Metabolites of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS)

Analysis of Microbial Siderophores by Mass Spectrometry

HSD18B7 Enzyme Assay Technique using a Triple Quadrupole Mass Spectrometer

Assays for Intracellular Cyclic Adenosine Monophosphate (cAMP) and Lysosomal Acidification

Isotopic Ratio Outlier Analysis (IROA) of Aqueous Humor for Metabolites

Assessment of Transport of Lipid Metabolites within Trabecular Meshwork Cells

Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes

Labeling Cancer Stem Cells with 13C6 Glucose and 13C5 Glutamine for Metabolic Flux Analysis

Protocols for Metabolomic Analyses from Tissues in Parkinson's Disease

Application of Stable Isotope Labels for Metabolomics in Studies in Fatty Liver Disease

Proteometabolomics of Melphalan Resistance in Multiple Myeloma

Metabolomic Analyses of Muscle Atrophy Induced by Hindlimb Unloading

Protocols for NMR Analysis in Livestock Metabolomics

Metabolomics Applicable to Retinal Vascular Diseases.

Other Format:

Printed edition:

ISBN:

978-1-4939-9488-5

9781493994885

Access Restriction:

Restricted for use by site license.