Protein Acetylation : Methods and Protocols / edited by Robert M. Brosh, Jr.

Format:

Book

Contributor:

Brosh, Jr., Robert M., editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1983.

Methods in Molecular Biology, 1064-3745 ; 1983

Language:

English

Subjects (All):

Biochemistry.

Gene expression.

Post-translational modification.

Protein Science.

Gene Expression.

Posttranslational Modification.

Local Subjects:

Protein Science.

Gene Expression.

Posttranslational Modification.

Physical Description:

1 online resource (XIII, 310 pages) : 52 illustrations, 40 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

The book focuses on the regulation of protein function and biological activity by the post-translational modification known as acetylation. Chapters detail a wide range of topics in protein acetylation dealing with pathways relevant to cellular homeostasis, gene expression, and DNA metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Acetylation: Methods and Protocols aims to be an extremely valuable resource to novices and experts alike whose research takes them in the direction of characterizing regulatory switches in protein acetylation events, and how they are manifested in significant ways in diverse mechanisms biological systems.

Contents:

High-Resolution Mass Spectrometry to Identify and Quantify Acetylation Protein Targets

Isolation of Proteins on Nascent Chromatin and Characterization by Quantitative Mass Spectrometry

Detection and Quantitation of Acetylated Histones on Replicating DNA using In Situ Proximity Ligation Assay and Click-it Chemisty

Quantification of In Vitro Protein Lysine Acetylation by Reversed Phase HPLC

Measurement and Analysis of Lysine Acetylation by Kat Complexes In Vitro and In Vivo

Site-Specific Lysine Acetylation Stoichiometry across Subcellular Compartments

Lysine Acetylation of Proteins and its Characterization in Human Systems

Molecular and Cellular Characterization of SIRT1 Allosteric Activators

Assessment of SIRT2 Inhibitors in Mouse Models of Cancer

Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision Repair

Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes

Analysis of DNA Processing Enzyme FEN1 and its Regulation by Protein Lysine Acetylation

Examining the Role of HDACs in DNA Double-Strand Break Repair in Neurons

Experimental Approaches to Investigate the Role of Helicase Acetylation in Regulating R-Loop Stability

Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling

Isolation and Quantification Brain Region-Specific and Cell Subtype-Specific Histone (De)Acetylation in Cognitive Neuroepigenetics

Functional Analysis of HDACs in Tumorigeneis.

Other Format:

Printed edition:

ISBN:

978-1-4939-9434-2

9781493994342

Access Restriction:

Restricted for use by site license.