Skin Tissue Engineering : Methods and Protocols / edited by Sophie Böttcher-Haberzeth, Thomas Biedermann.

Format:

Book

Contributor:

Böttcher-Haberzeth, Sophie, 1976- editor.

Biedermann, Thomas, 1979- editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1993.

Methods in Molecular Biology, 1064-3745 ; 1993

Language:

English

Subjects (All):

Cell culture.

Regenerative Medicine/Tissue Engineering.

Cell Culture.

Local Subjects:

Regenerative Medicine/Tissue Engineering.

Cell Culture.

Physical Description:

1 online resource (XII, 263 pages) : 79 illustrations, 66 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.

Contents:

Part 1 : Isolation and culture of skin cells

Chap 1: Isolation and Culture of Human Keratinocytes

Chap 2 : Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture

Chap 3 : Isolation and Culture of Epidermal Melanocytes

Chap 4: Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor

Chap 5 : Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells

Chap 6 : Isolation and Culture of Human Dermal Fibroblasts

Chap 7: Isolation and Culture of Human Dermal Microvascular Endothelial Cells

Chap 8: Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue

Part 2 : Tissue engineering skin and engineered skin models

Chap 9 : Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility

Chap 10 : Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes

Chap 11 : Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells

Chap 12 : Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes

Chap 13 : Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering

Chap 14 : Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models

Part 3 : In vitro and vivo models testing skin, skin cells, and tissue engineered skin

Chap 15 : Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts

Chap 16 : Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy

Chap 17 : Methods for Assessing Scaffold Vascualarization In Vivo

Chap 18 : Human Reconstructed Skin in a Mouse Model

Chap 19 : Pig Model to Test Tissue Engineered Skin

Chap 20 : Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model.

Other Format:

Printed edition:

ISBN:

978-1-4939-9473-1

9781493994731

Access Restriction:

Restricted for use by site license.