Enzyme-Mediated Ligation Methods / edited by Timo Nuijens, Marcel Schmidt.

Format:

Book

Contributor:

Nuijens, Timo, editor.

Schmidt, Marcel, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 2012.

Methods in Molecular Biology, 1064-3745 ; 2012

Language:

English

Subjects (All):

Biotechnology.

RNA-ligand interactions.

Enzymes.

Protein-Ligand Interactions.

Enzymology.

Local Subjects:

Biotechnology.

Protein-Ligand Interactions.

Enzymology.

Physical Description:

1 online resource (XI, 371 pages) : 94 illustrations, 59 illustrations in color.

Edition:

First edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana, 2019.

System Details:

text file PDF

Summary:

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. .

Contents:

Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody-Drug Conjugates

Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)

Cyclizing Disulfide-Rich Peptides using Sortase A

Chemo-Enzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides using Omniligase-1

Site-Specific Labeling of Proteins using the Formylglycine-Generating Enzyme (FGE)

Butelase 1-Mediated Ligation of Peptides and Proteins

Trypsiligase-Catalyzed Peptide and Protein Ligation

Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase

Tailoring Activity and Selectivity of Microbial Transglutaminase

SpyLigase-Catalyzed Modification of Antibodies

Peptide Cyclization Catalyzed by Cyanobactin Macrocyclases

In Vitro and In Planta Cyclization of Target Peptides using an Asparaginyl Endopeptidase from Oldenlandia affinis

Site-Specific Antibody Labeling Using Phosphopantetheinyl Transferase-Catalyzed Ligation

Lipoic Acid Ligase-Promoted Bioorthogonal Protein Modification and Immobilization

BioID as a Tool for Protein Proximity Labeling in Living Cells

N-Myristoyl transferase (NMT)-Catalyzed Labeling of Bacterial Proteins for Imaging in Fixed and Live Cells

Tubulin Tyrosine Ligase-Mediated Modification of Proteins

Inducible, Selective Labeling of Proteins via Enzymatic Oxidation of Tyrosine.

Other Format:

Printed edition:

ISBN:

978-1-4939-9546-2

9781493995462

Access Restriction:

Restricted for use by site license.