Enzyme kinetics and mechanism. Part E, Energetics of enzyme catalysis / edited by Vern L. Schramm, Daniel L. Purich.

Format:

Book

Contributor:

Schramm, Vern L.

Purich, Daniel L.

Series:

Methods in enzymology ; v. 308.

Methods in enzymology ; v. 308

Language:

English

Subjects (All):

Enzyme kinetics.

Dynamics.

Physical Description:

1 online resource (494 p.)

Other Title:

Energetics of enzyme catalysis

Place of Publication:

San Diego : Academic Press, c1999.

Language Note:

English

Summary:

This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications

Contents:

Front Cover; Enzyme Kinetics and Mechanism; Copyright Page; Table of Contents; Contributors to Volume 308; Preface; Volumes in Series; Section I: Energetic Coupling in Enzymatic Reactions; Chapter 1. Energetics of Substrate Binding, Catalysis, and Product Release; Chapter 2. Energy Coupling Through Molecular Discrimination: Nicotinate Phosphoribosyltransferase; Chapter 3. On the Advantages of Imperfect Energetic Linkage; Chapter 4. Reaction Dynamics of G-Protein Catalyzed Hydrolysis of GTP as Viewed by X-Ray Crystallographic Snapshots of Gia 1

Chapter 5. Energetics of Nucleotide Hydrolysis in Polymer Assembly/Disassembly: The Cases of Actin and TubulinChapter 6. Fundamental Mechanisms of Substrate Channeling; Section II: Intermediates and Complexes in Catalysis; Chapter 7. Intermediates and Energetics in Pyruvate Phosphate Dikinase; Chapter 8. Raman Spectroscopic Studies of the Structures, Energetics, and Bond Distortions of Substrates Bound to Enzymes; Chapter 9. Crystallographic Analysis of Solvent-Trapped Intermediates of Chymotrypsin; Section III: Detection and Properties of Low-Barrier Hydrogen Bonds

Chapter 10. Nuclear Magnetic Resonance Methods for the Detection and Study of Low-Barrier Hydrogen Bonds of EnzymesChapter 11. Hydrogen Bonding in Enzymatic Catalysis: Analysis of Energetic Contributions; Chapter 12. Application of Marcus Rate Theory to Proton Transfer in Enzyme-Catalyzed Reactions; Section IV: Transition State Determination and Inhibitors; Chapter 13. Enzymatic Transition-State Analysis and Transition-State Analogues; Chapter 14. Determining Transition States from Kinetic Isotope Effects; Chapter 15. Computational Methods for Transition State and Inhibitor Recognition

Author IndexSubject Index; Color Plate Section

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

ISBN:

9786611010959

9781281010957

1281010952

9780080496665

0080496660

OCLC:

476067754