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Guide to protein purification / edited by Murray P. Deutscher.
Holman Biotech Commons - Periodicals QP601 .M49 v.1 (1955)-v.148 (1987), v.150 (1987)-v.224 (1993), v.226 (1993)-v.228 (1993), v.230 (1994)-v.242 (1994), v.244 (1994)-v.253 (1995), v.257 (1996)-v.262 (1995), v.266 (1996)-v.284 (1997), v.286 (1997)-v.305 (1999), v.332 (2001)-v.333 (2001), v.341 (2001)-v.342 (2001) v.120 1986 (cum. index to v.81-94, 96-101) v.140 1988 (cum. index to v.102-119, 121-134) v.175 1990 (cum. index to v.135-139, 141-167) v.199 1993 (cum. index to v.168-174, 176-194) v.229 1995 (cum. index to v.195-198, 200-227) v.265 1996 (cum. index to v.228, 230-262) v.285 1998 (cum. index to v.263, 264, 266-289)
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Chemistry Library - Books QP601 .M49 v.1-v.193,v.195-v.207,v.209-v.220,v.222-v.228,v.230-v.259,v.261-v.263,v.265-v.285 v.286-v.365,v.367,v.369-v.654,v.656,v.658-v.725 v.168/174,176/194-v.195/198,200/227
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LIBRA QP601 .M49 v.1-v.217, v.219-v.220, v.222-v.288, v.291, v.299, v.303 v.306 (2000)-v.321 (2000) v.323 (2000)-v.331 (2001), v.334 (2001)-v.340 (2001), v.343 (2002)-v.390 (2004) v.320 2000 (cum. index to v.290-319) v.355 2002 (cum. index to v.321-354)
Mixed Availability
- Format:
- Book
- Series:
- Methods in enzymology ; 0076-6879 v. 182.
- Methods in enzymology, 0076-6879 ; v. 182
- Language:
- English
- Subjects (All):
- Proteins--Purification.
- Proteins.
- Proteins--isolation & purification.
- protein.
- Medical Subjects:
- Proteins--isolation & purification.
- Physical Description:
- 1 online resource (xxix, 894 pages) : illustrations
- Place of Publication:
- San Diego : Academic Press, ©1990.
- System Details:
- Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212
- Summary:
- Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
- Contents:
- Why purify enzymes / Arthur Kornberg
- Strategies and considerations for protein purifications / Stuart Linn
- Setting up a laboratory / Murray P. Deutscher
- Buffers: Principles and practice / Vincent S. Stoll, John S. Blanchard
- Measurement of enzyme activity / Edward F. Rossomando
- Quantitation of protein / Christa M. Stoscheck
- Concentration of proteins and removal of solutes / Thomas Pohl
- Maintaining protein stability / Murray P. Deutscher
- Overproduction of proteins in Escherichia coli: Vectors, hosts, and strategies / Asis Das
- Overexpression of proteins in eukaryotes / Margaret K. Bradley
- Secretion of heterologous proteins in Escherichia coli / I. Barry Holland, Brendan Kenny, Boris Steipe, Andreas Plückthun
- Preparation of extracts from prokaryotes / Millard Cull, Charles S. McHenry
- Preparation of extracts from yeast / S. Michal Jazwinski
- Preparation of Extracts from Plants / Peter Gegenheimer
- Preparation of extracts from higher eukaryotes / John David Dignam
- Isolation of subcellular organelles / Brian Storrie, Edward A Madden
- Preparation of membrane fractions / Juris Ozols
- Detergents: An overview / Judith M. Neugebauer
- Solubilization of native membrane proteins / Leonard M. Hjelmeland
- Solubilization of protein aggregates / Fiona A.O. Marston, Donna L. Hartley
- Removal of detergents from membrane proteins / Leonard M. Hjelmeland
- Precipitation techniques / Sasha Englard, Sam Seifter
- Precipitation of proteins with polyethylene glycol / Kenneth C. Ingham
- Ion-exchange chromatography / Edward F. Rossomando
- Gel filtration / Earle Stellwagen
- Protein chromatography on hydroxyapatite columns / Marina J. Gorbunoff
- Hydrophobic chromatography / Robert M. Kennedy
- Chromatography on immobilized reactive dyes / Earle Stellwagen
- Affinity chromatography: General methods / Steven Ostrove
- Affinity chromatography: Specialized techniques / Steven Ostrove, Shelly Weiss
- Chromatofocusing / Lallan Giri
- High-performance liquid chromatography: Effective protein purification by various chromatographic modes / Roman M. Chicz, Fred E. Regnier
- One-dimensional gel electrophoresis / David E. Garfin
- Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis / Bonnie S. Dunbar, Hitomi Kimura, Therese M. Timmons
- Isoelectric focusing / David E. Garfin
- Gel-staining techniques / Carl R. Merril
- Elution of protein from gels / Michael G. Harrington
- Purification of membrane proteins / Thomas C. Thomas, Mark G. McNamee
- Purification of DNA-binding proteins by site-specific DNA affinity chromatography / Shlomo Eisenberg, Stephen C. Francesconi, Chris Civalier, Scott S. Walker
- Purification of glycoproteins / Craig Gerard
- Purification of multienzyme complexes / Paul A. Srere, Christopher K. Mathews
- Determination of purity / David G. Rhodes, Thomas M. Laue
- Determination of size, molecular weight, and presence of subunits / Thomas M. Laue, David G. Rhodes
- Amino acid analysis / Juris Ozols
- Limited N-terminal sequence analysis / Paul Matsudaira
- Peptide mapping / Ralph C. Judd
- Analysis for protein modifications and nonprotein cofactors / Sam Seifter, Sasha Englard
- Protein crystallization / David Ollis, Stephen White
- Preparation of polyclonal antibodies / Bonnie S. Dunbar, Eric D. Schwoebel
- Preparation of monoclonal antibodies / Bonnie S. Dunbar, Sheri M. Skinner
- Protein blotting and immunodetection / Therese M. Timmons, Bonnie S. Dunbar
- Immunoprecipitation of proteins / Gary L. Firestone, Sandra D. Winguth
- Immunoassays / Charles W. Parker
- Radiolabeling of proteins / Charles W. Parker
- Using purified protein to clone its gene / John M. Wozney
- Computer analysis of protein structure / Patrick Argos
- Rethinking your purification procedure / Murray P. Deutscher
- The private life of DNA polymerase I / Arthur Kornberg
- Purification and crystallization of transaldolase from Candida utilis / B.L. Horecker, O. Tsolas
- Discovery of glutamine synthetase cascade / Earl R. Stadtman
- Discovery, resolution, purification, and function of elongation factors / Kivie Moldave.
- Notes:
- Includes bibliographical references and indexes.
- Print version record.
- Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2011.
- Other Format:
- Print version: Guide to protein purification.
- ISBN:
- 0121820831
- 9780121820831
- OCLC:
- 326630732
- Publisher Number:
- (WaSeSS)ssj0000492525
- Access Restriction:
- Restricted for use by site license
- Use copy Restrictions unspecified
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