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Pre-Clinical Models : Techniques and Protocols / edited by Paul C. Guest.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Guest, Paul C., editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 1916.
Methods in Molecular Biology, 1064-3745 ; 1916
Language:
English
Subjects (All):
Toxicology.
Laboratory animals.
Pharmacology/Toxicology.
Animal Models.
Local Subjects:
Pharmacology/Toxicology.
Animal Models.
Physical Description:
1 online resource (XIII, 343 pages) : 90 illustrations, 73 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2019.
System Details:
text file PDF
Summary:
This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.
Contents:
A Guide to Mass Spectrometry-based Quantitative Proteomics
Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors
Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes
The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents
The Forced Swim Test for Depression-like Behaviour in Rodents
Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice
The Nest Building Test in Mice for Assessment of General Well-being
Mouse Free Dyadic Social Interaction Test
The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour
The Y-maze for Assessment of Spatial Working and Reference Memory in Mice
Maturation of a Human Oligodendrocyte Cell Line
Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan
Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur
Characterization of an Animal Model of Autism and Social Interaction
Assaying Reproductive Capacity in Female Rodents
Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents
An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry
Characterization of the db/db Mouse Model of Type 2 Diabetes
Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes
Characterization of Human Transplantable Insulinoma Cells
Preparation of Islets from Rat Pancreas and Assessment of Islet Function
Cryopreservation of Red Blood Cells
Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation
Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors
The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis
Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research
The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity
OVA-induced Allergic Airway Inflammation Mouse Model
Description of Ovariectomy Protocol in Mice
A Rat Eye Lens Model of Cataract Formation
A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue
Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line- a Preclinical Model for Type 2 Diabetes.
Other Format:
Printed edition:
ISBN:
9781493989942
Access Restriction:
Restricted for use by site license.

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