Decellularized Scaffolds and Organogenesis : Methods and Protocols / edited by Kursad Turksen.

Format:

Book

Contributor:

Turksen, Kursad, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1577.

Methods in Molecular Biology, 1064-3745 ; 1577

Language:

English

Subjects (All):

Cytology.

Cell Biology.

Local Subjects:

Cell Biology.

Physical Description:

1 online resource (XVI, 347 pages) : 81 illustrations, 65 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Decellularized Scaffolds and Organogenesis: Methods and Protocols share novel approaches and insights that will provide new opportunities for those already in the field or moving to enter the field.

Contents:

Decellularization Methods for Scaffold Fabrication

Decellularized Liver Scaffold for Liver Regeneration

Wharton's Jelly Matrix Decellularization for Tissue Engineering Applications

3D-Decellularized Native Extra Cellular Matrix Scaffold for In Vitro Culture Expansion of Human Wharton Jelly Mesenchymal Stem Cells (hWJ MSCs)

Decellularized Iliotibial Band Recolonized with Allogenic Homotopic Fibroblasts or Bone Marrow-Derived Mesenchymal Stromal Cells

Preparation of Cell-Derived Decellularized Matrices Mimicking Native ECM During the Osteogenesis and Adipogenesis of Mesenchymal Stem Cells

Mouse Skeletal Muscle Decellularization

Extracellular Matrix from Whole Porcine Heart Decellularization for Cardiac Tissue Engineering

In-Lab Manufacturing of Decellularized Rat Renal Scaffold for Kidney Bioengineering

Glutaraldehyde Crosslinking Modification of Decellularized Rat Kidney Scaffolds

Preparation of Scaffolds from Decellularized Testicular Matrix

Decellularization of Bovine Small Intestinal Submucosa

Decellularization and Recellularization of Cartilage

A Protocol to Prepare Decellularized Stem Cell Matrix for Rejuvenation of Cell Expansion and Cartilage Regeneration

Tracheal Cartilage Isolation and Decellularization

Protocols for Rat Uterus Isolation and Decellularization: Applications for Uterus Tissue Engineering and 3D Cell Culturing

Application of Decellularized Tissue Scaffolds in Ovarian Tissue Transplantation

Composite Bioscaffolds Incorporating Decellularized ECM as a Cell-Instructive Component within Hydrogels as In Vitro Models and Cell Delivery Systems

3D-Printing Composite Polycaprolactone-Decellularized Bone Matrix Scaffolds for Bone Tissue Engineering Applications

Decellularization of Large Tendon Specimens: Combination of Manually Performed Freeze-Thaw Cycles and Detergent Treatment

Decellularized Bone Matrix Scaffold for Bone Regeneration

Preparation of Decellularized Liver Scaffolds and Recellularized Liver Grafts

Decellularization of Liver and Organogenesis in Rats

Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold

Liver Bioengineering Using Decellularized Whole-Liver Scaffolds

Decellularization of Intact Lung Tissue through Vasculature and Airways Using Negative and Positive Pressure

Alveolar Rhabdomyosarcoma Decellularization.

Other Format:

Printed edition:

ISBN:

9781493976560

Access Restriction:

Restricted for use by site license.