Microfluidic Electrophoresis : Methods and Protocols / edited by Debashis Dutta.

Format:

Book

Contributor:

Dutta, Debashis, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1906.

Methods in Molecular Biology, 1064-3745 ; 1906

Language:

English

Subjects (All):

Electrophoresis.

Biotechnology.

Local Subjects:

Electrophoresis.

Biotechnology.

Physical Description:

1 online resource (X, 255 pages) : 93 illustrations, 47 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2019.

System Details:

text file PDF

Summary:

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Contents:

Fabrication of Glass Microfluidic Devices

Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices

Sample Injection Techniques

Sample Preconcentration Protocols in Microfluidic Electrophoresis

Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

Micellar Electrokinetic Chromatography

Microchip Isotachophoresis: Analysis of Pharmaceuticals

Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination

Nanochannel Gradient Separations

Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications

Band Broadening Theories in Capillary Electrophoresis

Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation

Microchip Electrophoresis Tools for the Analysis of Small Molecules

Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out

Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips

Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow.

Other Format:

Printed edition:

ISBN:

9781493989645

Access Restriction:

Restricted for use by site license.