Systems Chemical Biology : Methods and Protocols / edited by Slava Ziegler, Herbert Waldmann.

Format:

Book

Contributor:

Ziegler, Slava, editor.

Waldmann, H. (Herbert), editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1888.

Methods in Molecular Biology, 1064-3745 ; 1888

Language:

English

Subjects (All):

Bioinformatics.

Local Subjects:

Bioinformatics.

Physical Description:

1 online resource (XI, 315 pages) : 65 illustrations, 43 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2019.

System Details:

text file PDF

Summary:

This volume explores the latest available wet-lab techniques and computational methods to study in-cell small-molecule behavior and interactions with their targets. The chapters in this book discuss topics such as disease-relevant models for chemical biology studies, target engagement using cellular thermal shift assay or bioluminescence resonance energy transfer; visualization of bio-active small molecules Raman microscopy; (phospho-)proteomics and transcriptomics for mode-of-action studies, CRISPR/Cas9-based chemogenomic profiling in mammalian cells; predicting drug interactions using computational approaches; comparison of compound-induced profiles using high-content imaging or cancer cell line panels and web-based tools for polypharmacology prediction. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Systems Chemical Biology: Methods and Protocols is a valuable resource for novice or expert scientists and researchers trying to initiate or continue their chemical biology studies at a systems level.

Contents:

1. PREDECT protocols for complex 2D/3D cultures

2. Phenotypic Screening using Mouse and Human Stem Cell-based Models of Neuroinflammation and Gene Expression Analysis to Study Drug Responses

3. Quantitative, Real-Time Measurements of Intracellular Target Engagement Using Energy Transfer

4. Target Engagement of Small Molecules - Thermal Profiling Approaches on Different Levels

5. Visualizing Bio-active Small Molecules by Alkyne-tagging and Slit-scanning Raman Microscopy

6. The Cell Painting Assay as a screening tool for the discovery of bioactivities in new chemical matter

7. Proteomic Profiling for Target Identification of Biologically Active Small Molecules using 2-D DIGE

8. Examining Cellular Responses to Kinase Drug Inhibition through Phosphoproteome Mapping of Substrates

9. CRISPR/Cas9-based Chemogenomic Profiling in Mammalian Cells

10. Exome Sequencing of Drug-resistant Clones for Target Identification

11. The use of large-scale chemically-induced transcriptome data acquired from LINCS to study small molecules

12. Integrated Analysis of Drug Sensitivity and Selectivity to Predict Synergistic Drug Combinations and Target Co-addictions in Cancer

13. Predicting Drug Interactions from Chemogenomics using INDIGO

14. Computational Analyses Connect Small-Molecule Sensitivity to Cellular Features Using Large Panels of Cancer Cell Lines

15. Web-based tools for Polypharmacology Prediction

16. In silico Target Prediction for Small Molecules.

Other Format:

Printed edition:

ISBN:

9781493988914

Access Restriction:

Restricted for use by site license.