Retinal Degeneration : Methods and Protocols / edited by Bernhard H. F. Weber, Thomas Langmann.

Format:

Book

Contributor:

Weber, Bernhard H. F., editor.

Langmann, Thomas, editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1834.

Methods in Molecular Biology, 1064-3745 ; 1834

Language:

English

Subjects (All):

Medicine.

Molecular Medicine.

Local Subjects:

Molecular Medicine.

Physical Description:

1 online resource (XIV, 417 pages) : 108 illustrations, 56 illustrations in color.

Edition:

Second edition 2019.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2019.

System Details:

text file PDF

Summary:

This volume provides key updates on several of the first edition chapters and also includes new novel techniques, addressing the most recent technological developments and their applications in retinal research. Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Contents:

Identification and Analysis of Genes Associated with Inherited Retinal Diseases

eQTL Analysis in Retinal Research

Testing for known Retinal Degeneration Mutants in Mouse Strains

CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis

Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells

Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture

Porcine RPE/Choroidal Explant Cultures

The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells

Cell Death Analysis in Retinal Cultures

Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease

Light Damage Models of Retinal Degeneration

Induction and Readout of Oxygen-Iinduced Retinopathy

Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9

Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases

Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa

Retinal Fundus Imaging in Mouse Models of Retinal Diseases

Phenotyping of Mouse Models with OCT

Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy

Immuno-TEM/STEM in Retinal Research

Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium

Cell-based Therapy for Retinal Disease: The New Frontier

In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy

Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo

Optimized Subretinal Injection Technique for Gene Therapy Approaches.

Other Format:

Printed edition:

ISBN:

9781493986699

Access Restriction:

Restricted for use by site license.