Protein Misfolding Diseases : Methods and Protocols / edited by Cláudio M. Gomes.

Format:

Book

Contributor:

Gomes, Cláudio M., 1970- editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1064-3745 ; 1873.

Methods in Molecular Biology, 1064-3745 ; 1873

Language:

English

Subjects (All):

Biochemistry.

Protein Science.

Local Subjects:

Protein Science.

Physical Description:

1 online resource (XIII, 338 pages) : 77 illustrations, 58 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2019.

System Details:

text file PDF

Summary:

This detailed book gathers a broad collection of experimental approaches to assist researchers in setting up different methods to investigate protein conformational disorders. Beginning with a section on assays focusing on biophysical approaches to study protein (mis)folding, the volume continues with sections on cellular and proteostasis assays as well as assays for protein folding correction and recovery, combining methods such as thermal shift assays, in silico improvement of protein solubility, and compound screening, an important area of research as it may open avenues for therapeutic strategies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Misfolding Diseases: Methods and Protocols serves as an ideal guide for researchers seeking to advance our knowledge of protein conformational disorders.

Contents:

Biophysical and Spectroscopic Methods for Monitoring Protein Misfolding and Amyloid Aggregation

Ultrasensitive RT-QuIC Seed Amplification Assays for Disease-Associated Tau, α-Synuclein, and Prion Aggregates

Vesicle-Based Assays to Study Membrane Interactions of Amyloid Peptides

Differential Scanning Fluorimetry and Hydrogen Deuterium Exchange Mass Spectrometry to Monitor the Conformational Dynamics of NBD1 in Cystic Fibrosis

A Multipronged Method for Unveiling Subtle Structural-Functional Defects of Mutant Chaperone Molecules Causing Human Chaperonopathies

High-Throughput Microplate-Based Fluorescence Assays for Studying Stochastic Aggregation of Superoxide Dismutase-1

Methods for Structural Analysis of Amyloid Fibrils in Misfolding Diseases

Assays for Light Chain Amyloidosis Formation and Cytotoxicity

Monitoring Aggregate Clearance and Formation in Cell-Based Assays

Monitoring Proteome Stress in Live Cells Using HaloTag-Based Fluorogenic Sensor

Quantification of Protein Aggregates Using Bimolecular Fluorescence Complementation

Screening Protein Aggregation in Cells Using Fluorescent Labels Coupled to Flow Cytometry

Induction of Cu/Zn Superoxide Dismutase (SOD1) Aggregation in Living Cells

A Cell Model for HSP60 Deficiencies: Modeling Different Levels of Chaperonopathies Leading to Oxidative Stress and Mitochondrial Dysfunction

Super-Resolution Fluorescence Imaging of Mutant Huntingtin Aggregation in Cells

Thermal Shift and Stability Assays of Disease-Related Misfolded Proteins Using Differential Scanning Fluorimetry

Methods to Screen Compounds against Mutant p53 Misfolding and Aggregation for Cancer Therapeutics

Early Stage Discovery and Validation of Pharmacological Chaperones for the Correction of Protein Misfolding Diseases

Constructing Kinetically Controlled Denaturation Isotherms of Folded Proteins Using Denaturant-Pulse Chaperonin Binding

In Vitro Prion Amplification Methodology for Inhibitor Screening

SolubiS: Optimizing Protein Solubility by Minimal Point Mutations.

Other Format:

Printed edition:

ISBN:

9781493988204

Access Restriction:

Restricted for use by site license.