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Pancreatic Cancer : Methods and Protocols / edited by Gloria H. Su.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Su, Gloria H., editor.
SpringerLink (Online service)
Series:
Springer Protocols (Springer-12345)
Methods in molecular biology 1064-3745 ; 1882.
Methods in Molecular Biology, 1064-3745 ; 1882
Language:
English
Subjects (All):
Oncology.
Cancer Research.
Local Subjects:
Cancer Research.
Physical Description:
1 online resource (XII, 337 pages) : 51 illustrations, 40 illustrations in color.
Edition:
Third edition 2019.
Contained In:
Springer eBooks
Place of Publication:
New York, NY : Springer New York : Imprint: Humana Press, 2019.
System Details:
text file PDF
Summary:
This third edition volume expands on the previous editions with discussions on the latest developments in 2D- and 3D-organoid cultures that allow for the execution of precision medicine, and in vitro studies of tumor cell growth and invasion. The chapters in this book explore protocols for evaluating macropinocytosis, stress granules, autophagy, metabolic alterations, and reactive oxygen species, which have showed to be possible therapeutic opportunities. The protocols described in this book look at both human and animal models, ranging from in vitro and in vivo, and will aid in future research of pancreatic cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Pancreatic Cancer: Methods and Protocols, Third Edition is a valuable resource for scientists and researchers who are interested in learning more about ways to improving patient care for pancreatic cancer.
Contents:
Subtyping of IPMN
Clinical Management and Surgical Decision-Making of IPMN of the Pancreas
Develop Nomogram to Predict Malignancy of Intraductal Papillary Mucinous Neoplasm
Generation of Induced Pluripotent Stem Cell-Like Lines from Human Pancreatic Ductal Adenocarcinoma
Lineage Tracing of Primary Human Pancreatic Acinar and Ductal Cells for Studying Acinar to Ductal Metaplasia
Spheroid Culture of Human Pancreatic Ductal Cells to Reconstitute Development of Pancreatic Intraepithelial Neoplasia
Pancreatic Microtumors: A Novel D Ex Vivo Testing Platform
3D Models of Pancreatic Ductal Adenocarcinoma via Tissue Engineering
Generation and Culture of Human Pancreatic Ductal Adenocarcinoma Organoids from Resected Tumor Specimens
Generation and Culture of Tumor and Metastatic Organoids from Murine Models of Pancreatic Ductal Adenocarcinoma
An In Vitro Three-Dimensional Organotypic Model to Analyze Peripancreatic Fat Invasion in Pancreatic Cancer: A Culture System Based on Collagen Gel Embedding
Osteoclasts Differentiation Assay
Primary Cultures for Pancreatic Stellate Cells (PSCs)
Cytokine Profiling and Orthotopic Xenografing of Pancreatic Stellate Cells
Quantitative Method to Track Proteolytic Invasion in 3D Collagen
Detection and Quantification of Macropinosomes in Pancreatic Tumors
Evaluating Stress Granules in Pancreatic Cancer In Vitro and In Vivo
Methods for Monitoring Macroautophagy in Pancreatic Cancer Cells
Measurement of Reactive Oxygen Species by Fluorescent Probes in Pancreatic Cancer Cells
Evaluating the Metabolic Alterations in Pancreatic Cancer
Isolation of Extracellular Vesicles for Cancer Diagnosis and Functional Studies
Evaluation of Post-Transcriptional Gene Regulation in Pancreatic Cancer Cells: Studying RNA Binding Proteins and their mRNA Targets
Laser Capture Microdissection on Frozen Sections for Extraction of High-Quality Nucleic Acids
Statistical Methods for Identifying Biomarkers from miRNA Profiles of Cancers
Induction of Pancreatic Inflammation Accelerates Pancreatic Tumorigenesis in Mice
Pancreatic Acinar to Ductal Metaplasia and Pancreatic Cancer
Orthotopic Pancreatic Tumor Mouse Models of Liver Metastasis
Molecular and Physiological Evaluation of Pancreatic Cancer-Induced Cachexia.
Other Format:
Printed edition:
ISBN:
9781493988792
Access Restriction:
Restricted for use by site license.

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