Test No. 488: Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays / Organisation for Economic Co-operation and Development.

Format:

Book

Government document

Author/Creator:

Organisation for Economic Co-operation and Development

Contributor:

SourceOECD (Online service)

Series:

OECD Guidelines for the Testing of Chemicals Section 4 20745788.

OECD Guidelines for the Testing of Chemicals, Section 4 20745788.

Language:

English

Subjects (All):

Ecology.

Physical Description:

1 online resource (15 pages).

Place of Publication:

Paris : OECD Publishing, 2020.

System Details:

text file

Summary:

This Test Guideline describes an in vivo assay that detects chemicals that may induce gene mutations in somatic and germ cells. In this assay, transgenic rats or mice that contain multiple copies of chromosomally integrated plasmid or phage shuttle vectors are used. The transgenes contain reporter genes for the detection of various types of mutations induced by test chemicals. A negative control group and a minimum of 3 treatment groups of transgenic animals are treated for 28 consecutive days. Administration is followed by a period of time, prior to sacrifice, during which the agent is not administered and during which unrepaired DNA lesions are fixed into stable mutations. At the end of this period (usually 3 or 28 days depending on the cell type and requirements), the animals are sacrificed, genomic DNA is isolated from the tissue(s) of interest and purified. Mutations that have arisen during treatment are scored by recovering the transgene and analysing the phenotype of the reporter gene in a bacterial host deficient for the reporter gene. Mutant frequency, the reported parameter in these assays, is calculated by dividing the number of plaques/plasmids containing mutations in the transgene by the total number of plaques/plasmids recovered from the same DNA sample.

Notes:

Title from title screen (viewed May 1, 2017).

ISBN:

9789264203907

Access Restriction:

Restricted for use by site license.