Time-resolved mass spectrometry : from concept to applications / Pawel Lukasz Urban, Yu-Chie Chen, Yi-Sheng Wang.

Format:

Book

Author/Creator:

Urban, Pawel, author.

Chen, Yu-Chie, author.

Wang, Yi-Sheng, author.

Language:

English

Subjects (All):

Mass spectrometry.

Spectrum analysis.

Physical Description:

1 online resource (388 pages)

Edition:

1st ed.

Place of Publication:

Chichester, West Sussex : Wiley, 2016.

Language Note:

English

Summary:

Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches - introduced during the past few decades - have shown temporal resolutions ranging from hours down to microseconds and beyond. Thistext covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS - in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling. It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.

Contents:

Intro

Title Page

Copyright

Table of Contents

Author Biographies

Preface

Acknowledgments

List of Acronyms

Chapter 1: Introduction

1.1 Time in Chemistry

1.2 Mass Spectrometry

1.3 Time-resolved Mass Spectrometry

1.4 Dynamic Matrices

1.5 Real-time vs. Single-point Measurements

1.6 Further Reading

References

Chapter 2: Ion Sources for Time-resolved Mass Spectrometry

2.1 Electron Ionization

2.2 Chemical Ionization

2.3 Atmospheric Pressure Chemical Ionization

2.4 Electrospray Ionization

2.5 Atmospheric Pressure Photoionization

2.6 Desorption/Ionization

2.7 Innovations in the 21st Century

2.8 Concluding Remarks

Chapter 3: Mass Analyzers for Time-resolved Mass Spectrometry

3.1 Overview

3.2 Individual Mass Analyzers

3.3 Integrated Analytical Techniques

Chapter 4: Interfaces for Time-resolved Mass Spectrometry

4.1 Molecules in Motion

4.2 Time-resolved Mass Spectrometry Systems

4.3 Concluding Remarks

Chapter 5: Balancing Acquisition Speed and Analytical Performance of Mass Spectrometry

5.1 Overview

5.2 Spectrum Acquisition Speed

5.3 Relationship between Spectrum Acquisition Time and Mass Spectrometer Performance

Chapter 6: Hyphenated Mass Spectrometric Techniques

6.1 Introduction

6.2 Separation Techniques Coupled with Mass Spectrometry

6.3 Ion-mobility Spectrometry

6.4 Other Hyphenated Systems

6.5 Influence of Data Acquisition Speed

6.6 Concluding Remarks

Chapter 7: Microfluidics for Time-resolved Mass Spectrometry

7.1 Overview

7.2 Fabrication

7.3 Microreaction Systems

7.4 Hydrodynamic Flow

7.5 Coupling Microfluidics with Mass Spectrometry

7.6 Examples of Applications

7.7 Digital Microfluidics

7.8 Concluding Remarks

References.

Chapter 8: Quantitative Measurements by Mass Spectrometry

8.1 The Challenge of Quantitative Mass Spectrometry Measurements

8.2 Selection of Instrument

8.3 Solutions to Quantitative Mass Spectrometry

8.4 Data Treatment

8.5 Concluding Remarks

Chapter 9: Data Treatment in Time-resolved Mass Spectrometry

9.1 Overview

9.2 Definition of Terms

9.3 Spectral Patterns

9.4 Mass Accuracy

9.5 Structural Derivation

9.6 Molecule Abundance

9.7 Time-dependent Data Treatment

Chapter 10: Applications in Fundamental Studies of Physical Chemistry

10.1 Overview

10.2 Chemical Kinetics

10.3 Chemical Equilibrium

Chapter 11: Application of Time-resolved Mass Spectrometry in the Monitoring of Chemical Reactions

11.1 Organic Reactions

11.2 Catalytic Reactions

11.3 Photochemical Reactions

11.4 Concluding Remarks

Chapter 12: Applications of Time-resolved Mass Spectrometry in the Studies of Protein Structure Dynamics

12.1 Electrospray Ionization in Protein Studies

12.2 Mass Spectrometry Strategies for Ultra-fast Mixing and Incubation

12.3 Hydrogen/Deuterium Exchange

12.4 Photochemical Methods

12.5 Implementation of Ion-mobility Spectrometry Coupled with Mass Spectrometry

12.6 Concluding Remarks

Chapter 13: Applications of Time-resolved Mass Spectrometry in Biochemical Analysis

13.1 Enzymatic Reactions

13.2 Time-resolved Mass Spectrometry in Systems and Synthetic Biology

13.3 Monitoring Living Systems

13.4 Concluding Remarks

Chapter 14: Final Remarks

14.1 Current Progress

14.2 Instrumentation

14.3 Software

14.4 Limitations

Index

End User License Agreement.

Notes:

Description based upon print version of record.

Description based on print version record.

ISBN:

9781118887356

1118887352

9781118887332

1118887336

9781118887349

1118887344

OCLC:

935920031