Capillary electrophoresis - mass spectrometry (CE-MS) : principles and applications / edited by Gerhardus de Jong.

Format:

Book

Contributor:

Jong, Gerhardus de, editor.

Language:

English

Subjects (All):

Capillary electrophoresis.

Mass spectrometry.

Physical Description:

1 online resource (421 p.)

Edition:

1st ed.

Place of Publication:

Weinheim, Germany : Wiley-VCH Verlag GmbH & Co. KGaA, 2016.

Summary:

This monograph offers the reader a complete overview on both principles and applications of CE-MS. Starting with an introductory chapter on detection in CE, also related and more specialized techniques such as electrophoretic and chromatographic preconcentration are discussed. A special emphasis is put on CE-MS interfaces, which are described in detail. In a separate chapter, attention is paid to sheath-liquid interfacing. The developments and possibilities of microchip CE-MS are also described. Applications to all relevant areas are discussed in distinct chapters, each written by experts in the respective fields. Besides applications in pharmaceutical analysis and bioanalysis, recent implementations in food science, forensic analysis, analysis of intact proteins, metabolomics and proteomics are highlighted. MS is a perfectly appropriate detection system for CE, as efficient separation is coupled to sensitive and selection detection. Moreover, MS can provide structure information on the separated compounds. CE-MS has now been developed into a strong hyphenated system complementary to LC-MS. This monograph is an unique source of knowledge for everyone dealing with and interested in CE-MS.

Contents:

Intro

Title Page

Copyright

Table of Contents

List of Contributors

Chapter 1: Detection in Capillary Electrophoresis - An Introduction

1.1 UV Absorption

1.2 Fluorescence

1.3 Conductivity

1.4 Mass Spectrometry

References

Chapter 2: Electrospray Ionization Interface Development for Capillary Electrophoresis-Mass Spectrometry

2.1 A Brief Introduction to the Development of CE-MS

2.2 Fundamentals of ESI and Electrochemical Reactions in CE-MS

2.3 Interface Designs

2.4 Specific Interface Applications

2.5 Conclusion

Abbreviations

Acknowledgments

Chapter 3: Sheath Liquids in CE-MS: Role, Parameters, and Optimization

3.1 Introduction

3.2 Sheath-Liquid Functions and Sheath-Flow Interface Design

3.3 Sheath-Liquid-Related Parameters and their Selection

3.4 Sheath Liquids for Non-ESI CE-MS Interfaces

3.5 Sheath-Flow Chemistry

3.6 Conclusions

Chapter 4: Recent Developments of Microchip Capillary Electrophoresis Coupled with Mass Spectrometry

4.1 Introduction

4.2 Microchip Capillary Electrophoresis

4.3 Reviews on MCE and MCE-MS

4.4 Principal Requirements for MCE-MS

4.5 MCEMS by Direct Off-Chip Spraying

4.6 MCE-MS with Connected Sprayer

4.7 MCE-MS Devices with Integrated Sprayer

4.8 Multidimensional MCE-MS Devices

4.9 Conclusions and Perspectives

Appendix

Chapter 5: On-Line Electrophoretic, Electrochromatographic, and Chromatographic Sample Concentration in CE-MS

5.1 Introduction

5.2 Electrophoretic and Electrochromatographic Sample Concentration or Stacking

5.3 On-line/In-line SPE with CE-MS

5.4 Conclusion

Acknowledgment

Chapter 6: CE-MS in Drug Analysis and Bioanalysis

6.1 Introduction

6.2 CE-MS in Drug Analysis

6.3 CE-MS in Bioanalysis.

6.4 CE-MS in Drug Metabolism Studies

6.5 Quantitative Aspects in CE-MS

6.6 Conclusions

Chapter 7: CE-MS for the analysis of intact proteins

7.1 Introduction

7.2 CE of Intact Proteins

7.3 MS Detection of Intact Proteins

7.4 Applications of Intact Protein CE-MS

7.5 Conclusions

Chapter 8: CE-MS in Food Analysis and Foodomics

8.1 Introduction: CE-MS, Food Analysis, and Foodomics

8.2 Concluding Remarks

Chapter 9: CE-MS in Forensic Sciences with Focus on Forensic Toxicology

9.1 Introduction

9.2 Sample Preparation of Forensically Relevant Matrices

9.3 Separation Modes and Analytical Conditions

9.4 Applications

9.5 Conclusions

Chapter 10: CE-MS in Metabolomics

10.1 Introduction

10.2 Sample Preparation and MS Systems

10.3 Application

10.4 Conclusions

Chapter 11: CE-MS for Clinical Proteomics and Metabolomics: Strategies and Applications

11.1 Introduction

11.2 Clinical Proteomics

11.3 Clinical Metabolomics

11.4 Conclusions and Perspectives

Index

End User License Agreement.

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on online resource; title from PDF title page (ebrary, viewed July 8, 2016).

ISBN:

3-527-69383-1

3-527-69381-5

3-527-69380-7

OCLC:

952248078