An introduction to high content screening : imaging technology, assay development, and data analysis in biology and drug discovery / editors, Steven A. Haney [and four others].

Format:

Book

Contributor:

Haney, Steven A., editor.

Language:

English

Subjects (All):

Drugs--Design.

Drugs.

Systems biology.

Physical Description:

1 online resource (742 p.)

Edition:

1st ed.

Place of Publication:

Hoboken, New Jersey : Wiley, 2015.

Summary:

<br />Using a collaborative and interdisciplinary author base withexperience in the pharmaceutical industry and academia, this bookis a practical resource for high content (HC) techniques. Instructs readers on the fundamentals of highcontent screening (HCS) techniques<br /> Focuses on practical and widely-used techniques likeimage processing and multiparametric assays<br /> Breaks down HCS into individual modules for trainingand connects them at the end<br /> Includes a tutorial chapter that works through sampleHCS assays, glossary, and detailed appendices

Contents:

Cover; Titlepage; Copyright; PREFACE; CONTRIBUTORS; 1 Introduction; 1.1 The Beginning of High Content Screening; 1.2 Six Skill Sets Essential for Running HCS Experiments; 1.3 Integrating Skill Sets Into A Team; 1.4 A Few Words on Experimental Design; 1.5 Conclusions; Key Points; Further Reading; References; SECTION I FIRST PRINCIPLES; 2 Fluorescence and Cell Labeling; 2.1 Introduction; 2.2 Anatomy of Fluorescent Probes, Labels, and Dyes; 2.3 Stokes' Shift and Biological Fluorophores; 2.4 Fluorophore Properties; 2.5 Localization of Fluorophores Within Cells

2.6 Multiplexing Fluorescent Reagents2.7 Specialized Imaging Applications Derived From Complex Properties of Fluorescence; 2.8 Conclusions; Key Points; Further Reading; References; 3 Microscopy Fundamentals; 3.1 Introducing HCS Hardware; 3.2 Deconstructing Light Microscopy; 3.3 Using the Imager to Collect Data; 3.4 Conclusions; Key Points; Further Reading; References; 4 Image Processing; 4.1 Overview of Image Processing and Image Analysis in HCS; 4.2 What is a Digital Image?; 4.3 "Addressing" Pixel Values in Image Analysis Algorithms; 4.4 Image Analysis Workflow; 4.5 Conclusions; Key Points

Further ReadingReferences; SECTION II GETTING STARTED; 5 A General Guide to Selecting and Setting Up A High Content Imaging Platform; 5.1 Determining Expectations of the Hcs System; 5.2 Establishing an Hc Platform Acquisition Team; 5.3 Basic Hardware Decisions; 5.4 Data Generation, Analysis, and Retention; 5.5 Installation; 5.6 Managing the System; 5.7 Setting Up Workflows for Researchers; 5.8 Conclusions; Key Points; Further Reading; 6 Informatics Considerations; 6.1 Informatics Infrastructure for High Content Screening; 6.2 Using Databases to Store HCS Data

6.3 Mechanics of an Informatics Solution6.4 Developing Image Analysis Pipelines: Data Management Considerations; 6.5 Compliance With Emerging Data Standards; 6.6 Conclusions; Key Points; Further Reading; References; 7 Basic High Content Assay Development; 7.1 Introduction; 7.2 Initial Technical Considerations for Developing a High Content Assay; 7.3 A Simple Protocol to Fix and Stain Cells; 7.4 Image Capture and Examining Images; 7.5 Conclusions; Key Points; Further Reading; Reference; SECTION III ANALYZING DATA; 8 Designing Metrics for High Content Assays

8.1 Introduction: Features, Metrics, Results8.2 Looking at Features; 8.3 Metrics and Results: The Metric is The Message; 8.4 Types of High Content Assays and Their Metrics; 8.5 Metrics to Results: Putting it all Together; 8.6 Conclusions; Key Points; Further Reading; References; 9 Analyzing Well-Level Data; 9.1 Introduction; 9.2 Reviewing Data; 9.3 Plate and Control Normalizations of Data; 9.4 Calculation of Assay Statistics; 9.5 Data Analysis: Hit Selection; 9.6 IC 50 Determinations; 9.7 Conclusions; Key Points; Further Reading; References; 10 Analyzing Cell-Level Data; 10.1 Introduction

10.2 Understanding General Statistical Terms and Concepts

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

1-118-85939-1

1-118-85947-2

OCLC:

903319000