Epigenetics and dermatology / Qianjin Lu, Christopher C. Chang and Bruce C. Richardson ; contributors Nezam Altorok [and fifty two others].

Format:

Book

Author/Creator:

Lu, Qianjin, author.

Chang, Christopher C., author.

Richardson, Bruce C., author.

Contributor:

Altorok, Nezam, contributor.

Language:

English

Subjects (All):

Epigenetics.

Skin--Diseases.

Skin.

Dermatology.

Physical Description:

1 online resource (525 p.)

Edition:

1st ed.

Place of Publication:

London, [England] : Academic Press, 2015.

Language Note:

English

Summary:

Epigenetics and Dermatology explores the role of epigenetics in the pathogenesis of autoimmune-related skin diseases and skin cancer. Leading contributors cover common and uncommon skin conditions in which extensive epigenetic research has been done. They explain how environmental exposures (chemicals, drugs, sunlight, diet, stress, smoking, infection, etc.) in all stages of life (from a fetus in-utero to an elderly person) may result in epigenetic changes that lead to development of some skin diseases in life. They also discuss the possibilities of new and emergent epigenetic treatments whic

Contents:

Front Cover; Epigenetics and Dermatology; Copyright Page; Dedication; Contents; List of Contributors; Preface; Acknowledgments; 1. Biological and Historical Aspects of Epigenetics; 1 Introduction to Epigenetics; References; 2 Laboratory Methods in Epigenetics; 2.1 Introduction; 2.2 DNA Methylation Analysis; 2.2.1 Methods to Distinguish 5-Methylcytosine from Cytosine; 2.2.1.1 Restriction Endonuclease-Based Analysis; 2.2.1.1.1 Southern Blot; 2.2.1.1.2 Methylation-Sensitive Amplified Polymorphism; 2.2.1.2 Bisulfite Conversion Technique and Derivatives; 2.2.1.2.1 Bisulfite Sequencing PCR

2.2.1.2.2 Pyrosequencing2.2.1.2.3 Combined Bisulfite and Restriction Analysis; 2.2.1.2.4 Methylation-Sensitive Single-Nucleotide Primer Extension and SnuPE Ion Pair Reversed-Phase High Performance Liqui...; 2.2.1.2.5 Methylation-Sensitive Melting Curve Analysis; 2.2.1.2.6 Methylation-Sensitive High-Resolution Melting; 2.2.1.2.7 MethyLight; 2.2.1.3 Immunoprecipitation-Based Methods; 2.2.1.3.1 Methylated-CpG Island Recovery Assay; 2.2.1.3.2 Methyl-Binding-PCR; 2.2.1.4 Mass Spectrometry-Based Methods; 2.2.1.4.1 MALDI-TOF Mass Spectrometry with Base-Specific Cleavage

2.2.1.4.2 MALDI-TOF Mass Spectrometry with Primer Extension2.2.2 Genome-Scale DNA Methylation Analysis; 2.2.2.1 Microarray-Based Analysis of DNA Methylation Changes; 2.2.2.1.1 Sample Preparation; 2.2.2.1.2 Microarray Used in DNA Methylation Profiling; 2.2.2.2 Next-Generation Sequencing Techniques; 2.3 Techniques Used for 5hmC Mark Detection; 2.4 Histone Modification Analysis; 2.4.1 Chromatin Immunoprecipitation; 2.4.2 ChIP-on-Chip; 2.4.3 ChIP-seq; 2.4.3.1 Workflow of ChIP-seq; 2.4.3.2 Analysis Pipeline of ChIP-seq Data; 2.4.3.2.1 Read Aligner; 2.4.3.2.2 Peak Calling; 2.4.3.2.3 Motif Finding

2.4.3.3 Advantages of ChIP-seq2.4.4 Challenges for Histone Modification Analysis; 2.5 miRNA Analysis; 2.5.1 miRNA Detection; 2.5.1.1 Microarray; 2.5.1.2 Next-Generation Sequencing; 2.5.1.3 RT-PCR; 2.5.1.4 Northern Blot Analysis; 2.5.1.5 Others; 2.5.2 Target Prediction; 2.5.2.1 Target Scan; 2.5.2.2 PicTar; 2.5.2.3 DIANA-microT; 2.5.2.4 Others; 2.5.3 Target Validation and Functional Analysis; 2.5.3.1 Luciferase Reporter Assays; 2.5.3.2 Gain-of-Function and Loss-of-Function Experiments; 2.6 Conclusion; List of Abbreviations; References; 3 Keratinocyte Differentiation and Epigenetics

3.1 Introduction3.2 Gene Expression in Keratinocyte Differentiation; 3.3 Epigenetic Modulation in Keratinocyte Differentiation; 3.4 Epigenetics and Skin Diseases; 3.5 Conclusion; References; 4 Epigenetics and Fibrosis: Lessons, Challenges, and Windows of Opportunity; 4.1 Introduction; 4.2 Incidence and Prevalence of Fibrosis; 4.3 Biology of Epigenetics; 4.3.1 DNA Methylation; 4.3.2 Histone Modifications; 4.4 Epigenetics and Fibrosis; 4.4.1 Epigenetics and Lung Fibrosis; 4.4.1.1 DNA Methylation and Lung Fibrosis; 4.4.1.2 Histone Modifications and Lung Fibrosis

4.4.2 Epigenetics and Liver Fibrosis

Notes:

Description based upon print version of record.

Includes bibliographical references at the end of each chapters and index.

Description based on online resource; title from PDF title page (ebrary, viewed February 28, 2015).

ISBN:

0-12-801272-2

OCLC:

904099983