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Principles and practicals in medical microbiology / Oto Melter, Annika Malmgren.
- Format:
- Book
- Author/Creator:
- Melter, Oto, author.
- Language:
- English
- Subjects (All):
- Medical microbiology.
- Microbiological techniques.
- Physical Description:
- 1 online resource (135 pages)
- Edition:
- First edition.
- Place of Publication:
- [Place of publication not identified] : Karolinum Press, [2014]
- Summary:
- The book has been created for medical students to ease the comprehension of the relations between theory and practice in current medical microbiology. The core comprehensive data are reviewed from prestigious publications in the field and professional experience of the authors. Each chapter contains a theoretical and a practical part. The practical part is divided into a few exercises which allows for the practice of some of the basic experimental procedures used in laboratories of medical microbiology. We believe that the original photographs and the diagrams that have been newly created by the authors will improve the understanding of the basic principles of microbiological diagnosis. Each chapter is complemented with a lab quiz intended to help students review their basic knowledge.
- Contents:
- Cover
- Contents
- Introduction
- 1 Laboratory safety rules
- 1.1 Purpose of the safety principles
- 1.2 Principles
- 2 Specimen collection &
- diagnostic principles
- 2.1 Specimen collection &
- transport
- 2.2 Material &
- methods
- 2.3 Conditions for specimen collection &
- 2.4 Request form
- 2.5 Diagnostic principles in medical microbiology
- 2.6 Light microscopy
- 2.7 Gram staining - basic staining procedure in clinical microbiology
- 2.7.1 Smear preparation
- 2.7.2 Quality of cell wall &
- Gram staining procedure
- 2.8 Practical part - specimen collection &
- 2.9 Lab quiz
- 3 Direct detection &
- typing of infectious agents
- 3.1 Definition of agent detection &
- typing
- 3.2 Application of microscopy technics
- 3.3 Application of nucleic acid based methods
- 3.3.1 Principle of DNA amplification by PCR methods
- 3.3.2 Principle of DNA hybridization methods
- 3.3.3 Principle of sequence based methods
- 3.4 Other methods in direct antigen detection
- 3.4.1 Immunochromatographic method
- 3.5 Practical part - direct detection &
- 3.6 Lab quiz
- 4 Cultivation of infectious agents
- 4.1 Cultivation - definition
- 4.2 Cultivation of bacterial, viral &
- parasitic agents
- 4.3 Media for bacterial cultivation
- 4.4 Examples of bacterial cultures
- 4.5 Conditions for cultivation
- 4.6 Special staining procedures
- 4.7 P ractical part - special stainings &
- cultivation
- 4.8 Lab quiz
- 5 Antibiotic susceptibility testing
- 5.1 Antimicrobials
- mode of action &
- resistance
- 5.2 Antimicrobial susceptibility testing
- 5.3 Basic methods
- 5.4 Interpretations of the results
- 5.5 Other methods
- procedures &
- interpretation
- 5.6 Practical part - special stainings &.
- cultivation of infectious agents
- 5.7 Lab quiz
- 6 Identification of infectious agents
- 6.1 Definition of identification of infectious agents
- 6.2 Phenotypical methods
- 6.3 Application of phenotypical methods
- 6.4 Genotypical methods &
- application
- 6.5 Practical part - identification of infectious agents
- 6.6 Lab quiz
- 7 Detection of specific antibodies
- 7.1 Immune response
- 7.2 Antigens &
- antibodies
- 7.3 Basic serological methods
- 7.4 Serological methods &
- 7.5 Practical part - detection of specific antibodies
- 7.6 Lab quiz
- 8 General mycology
- 8.1 Fungi
- 8.2 Classification
- 8.3 Diagnostics
- 8.3.1 Microscopy
- 8.3.2 Cultivation
- 8.3.3 Identification
- 8.4 Susceptibility to antimycotical drugs
- 8.5 Serological methods
- 8.6 Direct detection of the agent in clinical material
- 8.7 Practical part - general mycology
- 8.8 Lab quiz
- 9 General virology
- 9.1 Definition of viruses
- 9.2 History
- 9.3 Electronmicroscopic evidence
- 9.4 Virus detection by other methods
- 9.5 Praktical part - general virology
- 9.6 Lab quiz
- 10 General parasitology
- 10.1 Parasitism, parasite and host
- 10.2 Classification
- 10.3 Diagnostic methods
- 10.4 Material and diagnostics
- 10.5 Practical part - general parasitology
- 10.6 Lab quiz
- 11 Molecular microbiology &
- epidemiology
- 11.1 Definition
- 11.2 Bacterial cell wall synthesis and antibiotics
- 11.3 Antibiotic resistance genes
- 11.4 Regulation of structural genes for antibiotic resistance
- 11.5 Epidemiologic typing
- 11.6 Practical part - molecular microbiology &
- 11.7 Lab quiz
- 12 Staphylococci
- 12.1 General features
- 12.2 Virulence factors and pathogenesis
- 12.3 Infections
- 12.4 Treatment, prevention &
- control
- 12.5 Laboratory diagnosis.
- 12.6 Practical part - staphylococci
- 12.7 Lab quiz
- 13 Streptococci &
- enterococci
- 13.1 General features
- 13.2 Classification
- 13.3 Virulence factors and pathogenesis
- 13.4 Infections and poststreptococcal diseases
- 13.5 Treatment, prevention &
- 13.6 Laboratory diagnosis
- 13.7 Practical part - streptococci &
- 13.8 Lab quiz
- 14 Corynebacteria &
- listeria
- 14.1 General features
- 14.2 Virulence factors &
- pathogenesis
- 14.3 Infections &
- 14.4 Treatment, prevention &
- 14.5 Laboratory diagnosis
- 14.6 Practical part - corynebacteria &
- 14.7 Lab quiz
- 15 Enterobacteria &
- enteric pathogens
- 15.1 General features
- 15.2 Virulence factors &
- 15.3 Infections &
- 15.4 Treatment, prevention &
- 15.5 Laboratory diagnosis
- 15.6 Practical part - enterobacteria and enteric pathogens
- 15.7 Lab quiz
- 16 Anaerobic bacteria
- 16.1 General features
- 16.2 Virulence factors &
- 16.3 Infections &
- 16.4 Treatment, prevention &
- 16.5 Laboratory diagnosis
- 16.6 Practical part - anaerobic bacteria
- 16.7 Lab quiz
- 17 Neisseria, Bordetella &
- Haemophilus
- 17.1 General features
- 17.2 Virulence factors &
- 17.3 Infections &
- 17.4 Treatment, prevention &
- 17.5 Laboratory diagnosis
- 17.6 Practical part - Neisseria, Bordetella, Haemophilus
- 17.7 Lab quiz
- 18 Pseudomonas aeruginosa &
- other non-fermenters
- 18.1 General features
- 18.2 Virulence factors &
- 18.3 Infections &
- 18.4 Treatment, prevention &
- 18.5 Laboratory diagnosis.
- 18.6 Practical part - Pseudomonas and non-fermenters
- 18.7 Lab quiz
- 19 Mycobacteria
- 19.1 General features
- 19.2 Classification of mycobacteria
- 19.3 Virulence factors &
- 19.4 Infections &
- 19.5 Treatment, prevention &
- 19.6 Laboratory diagnosis
- 19.7 Practical part - mycobacteria
- 19.8 LAB QUIZ
- 20 Yeasts
- 20.1 General features
- 20.2 Virulence factors &
- 20.3 Infections &
- 20.4 Treatment, prevention &
- 20.5 Laboratory diagnosis
- 20.6 Practical part - yeasts
- 20.7 Lab quiz
- 21 Arthropods
- 21.1 General properties
- 21.2 Classification
- 21.3 Pathogenesis
- 21.4 Infecton caused or trasmitted by arthropods
- 21.5 Treatment and prevention
- 21.6 Laboratory diagnosis
- 21.7 Practical part - arthropods
- 21.8 Lab quiz.
- Notes:
- Description based on print version record.
- ISBN:
- 9788024625447
- 802462544X
- OCLC:
- 1043646675
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