Advances in botanical research ; v. 76 / Plant cyclotides / volume editor, David J. Craik.

Format:

Book

Contributor:

Craik, David, editor.

Series:

Advances in botanical research ; 0065-2296 v.76 (OCoLC)1461155

Advances in botanical research, 0065-2296 ; v. 76

Language:

English

Subjects (All):

Botany--Research.

Botany.

Plant molecular biology.

Plant proteomics.

Peptides.

Botanical chemistry.

Physical Description:

1 online resource (404 p.)

Edition:

First edition.

Place of Publication:

Amsterdam : Elsevier, 2015.

Summary:

Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 76th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology.- Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences- Contains commentary by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology- This volume features reviews of the fast moving field of plant cyclotides

Contents:

Chapter 1. Overview on the Discovery and Applications of Cyclotides

1. Introduction

Acknowledgments

References

Chapter 2. Cyclotides in the Violaceae

1. The discovery of cyclotides in violaceae

2. Unlocking the cyclotide library

3. The violaceae

4. Violaceae-the pharmacopoeia

5. Growing needs and sources of cyclotides

6. Completing the circle

Chapter 3. Cyclotides in the Rubiaceae

2. Discovery of cyclotides

2.1 Cyclotide Analytical Screening Workflow

2.2 Cyclotides Sequence Analysis Using Transcriptomics and Peptidomics

3. Distribution of cyclotides in rubiaceae

3.1 Rubiaceae-An Overview

3.2 Morphology and Taxonomy of Rubiaceae

3.3 Distribution of Cyclotides within Rubiaceae

3.4 Cyclotide Precursors of Rubiaceae Plants

3.5 Cyclotide Sequence Variations within Rubiaceae

3.6 Distribution of Cyclotides in Other Gentianales

4. Evolution of cyclotides

5. Bioactivities of rubiaceae cyclotides

6. Conclusion and outlook

Chapter 4. Cyclotides from Chinese Plants

2. Chemical Detection Method for Cyclotides

3. Distribution and Chemotaxonomy of Cyclotides in Chinese Plants

4. Extraction, Isolation and Structural Elucidation of Cyclotides in Chinese Plants

4.1 Extraction and Isolation of Cyclotides in Chinese Plants

4.2 Tandem MS Sequencing of Cyclotides in Chinese Plants

4.3 NMR Characterization of Cyclotides in Chinese Plants

5. Biological Activity of Cyclotides in Chinese Plants

Chapter 5. Primary Structural Analysis of Cyclotides

1. Cyclotide discovery-untangling the cystine knot

2. Tools for peptide sequence assignment

2.1 gene sequencing

2.2 Genome Mining

2.3 Next-Generation Sequencing

2.4 Peptide Extraction and Purification

2.5 Acid Hydrolysis and Amino Acid Analysis

2.6 Edman Degradation

2.7 Chemical and Enzymatic Digestion

3. Mass spectrometry

3.1 Ionization

3.1.1 Electrospray Ionization

3.1.2 Nanoelectrospray Ionization

3.1.3 Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

3.2 Mass Analyzers

3.2.1 Quadrupole Mass Analyzers

3.2.2 TOF Mass Analyzers

3.3 Peptide Sequencing

4. Application of mass spectrometry to cyclotide sequencing

4.1 MS Analysis of Cyclotides

4.2 Tandem Mass Spectrometric (MS/MS) Analysis of Cyclotides

4.3 Application of "Omics" Technologies to Cyclotide Sequencing

5. Challenges and complexities

5.1 Co-eluting, Isobaric Peptides

5.2 N/D Isoforms

5.3 Unknown Genomes/Incomplete DBs

6. Future prospects

6.1 Transcriptomics and Bioinformatics

6.2 Automation and Robotics

6.3 Advances in MS Hardware

6.4 Advances in MS Software

7. Concluding remarks

Chapter 6. Structural Studies of Cyclotides

1. The cyclic cystine knot

2. Structural features of cyclotides

2.1 Structural Studies

2.2 Möbius, Bracelets, and Trypsin Inhibitors

Chapter 7. Natural Functions and Structure–Activity Relationships of Cyclotides

2. Pesticidal Activities of Cyclotides

3. Pharmaceutical Activities of Cyclotides

4. Cytotoxic Activities of Cyclotides

5. Structure–Activity Relationships

6. Concluding Remarks

Chapter 8. Biosynthesis of Cyclotides

1. Introduction

2. Cyclotides are Gene Products

3. The Cyclotide Processing Pathway

4. Sequence Requirements for Cyclization

5. Circularization of Cyclotides is Mediated by a Vacuolar Processing Enzyme (VPE)

6. Vacuolar Processing Enzymes (VPEs)

7. Circularization of Other Backbone Cyclized Peptides

8. Conclusion

Chapter 9. Chemical and Biological Production of Cyclotides

Chapter 10. Cyclotides in a Biotechnological Context: Opportunities and Challenges1. Cyclotides in a Biotechnological Context

2. Cyclotide Production Systems

3. Market Analysis of Cyclotides in Plant Biotechnology

4. Future Prospects for Cyclotides and Plant Biotechnology

Index.

Notes:

Description based upon print version of record.

Includes bibliographical references and indexes.

Description based on online resource; title from PDF title page (ebrary, viewed December 31, 2015).

Description based on publisher supplied metadata and other sources.

ISBN:

0-12-800030-9

0-12-800797-4

OCLC:

930489358