Advanced surfaces for stem cell research / edited by Ashutosh Tiwari, Bora Garipcan and Lokman Uzun.

Format:

Book

Contributor:

Tiwari, Ashutosh, 1978- editor.

Garipcan, Bora, editor.

Uzun, Lokman, editor.

Series:

Advanced Material Series

Language:

English

Subjects (All):

Stem cells--Research.

Stem cells.

Cell membranes--Research.

Cell membranes.

Physical Description:

1 online resource (483 pages) : illustrations (some color), charts.

Edition:

1st ed.

Place of Publication:

Hoboken, New Jersey : Scrivener Publishing : Wiley, 2017.

Summary:

The book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book. This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including: * Extracellular matrix proteins for stem cell fate * The superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulator * Effects of mechanotransduction on stem cell behavior * Modulation of stem cells behavior through bioactive surfaces * Influence of controlled micro and nanoengineered surfaces on stem cell fate * Nanostructured polymeric surfaces for stem cells * Laser surface modification techniques and stem cells applications * Plasma polymer deposition: a versatile tool for stem cell research * Application of bioreactor concept and modeling techniques in bone regeneration and augmentation treatments * Substrates and surfaces for control of pluripotent stem cell fate and function * Application of biopolymer-based, surface modified devices in transplant medicine and tissue engineering * Silk as a natural biopolymer for tissue engineering

Contents:

Cover

Title Page

Copyright Page

Contents

Preface

1 Extracellular Matrix Proteins for Stem Cell Fate

1.1 Human Stem Cells, Sources, and Niches

1.2 Role of Extrinsic and Intrinsic Factors

1.2.1 Shape

1.2.2 Topography Regulates Cell Fate

1.2.3 Stiffness and Stress

1.2.4 Integrins

1.2.5 Signaling via Integrins

1.3 Extracellular Matrix of the Mesenchyme: Human Bone Marrow

1.4 Biomimetic Peptides as Extracellular Matrix Proteins

References

2 The Superficial Mechanical and Physical Properties of Matrix Microenvironment as Stem Cell Fate Regulator

2.1 Introduction

2.2 Fabrication of the Microenvironments with Different Properties in Surfaces

2.3 Effects of Surface Topography on Stem Cell Behaviors

2.4 Role of Substrate Stiffness and Elasticity of Matrix on Cell Culture

2.5 Stem Cell Fate Induced by Matrix Stiffness and Its Mechanism

2.6 Competition/Compliance between Matrix Stiffness and Other Signals and Their Effect on Stem Cells Fate

2.7 Effects of Matrix Stiffness on Stem Cells in Two Dimensions versus Three Dimensions

2.8 Effects of External Mechanical Cues on Stem Cell Fate from Surface Interactions Perspective

2.9 Conclusions

Acknowledgments

3 Effects of Mechanotransduction on Stem Cell Behavior

3.1 Introduction

3.2 The Concept of Mechanotransduction

3.3 The Mechanical Cues of Cell Differentiation and Tissue Formation on the Basis of Mechanotransduction

3.4 Mechanotransduction via External Forces

3.4.1 Mechanotransduction via Bioreactors

3.4.2 Mechanotransduction via Particle-based Systems

3.4.3 Mechanotransduction via Other External Forces

3.5 Mechanotransduction via Bioinspired Materials

3.6 Future Remarks and Conclusion

Declaration of Interest

4 Modulation of Stem Cells Behavior Through Bioactive Surfaces.

4.1 Lithography

4.2 Micro and Nanopatterning

4.3 Microfluidics

4.4 Electrospinning

4.5 Bottom-up/Top-down Approaches

4.6 Substrates Chemical Modifications

4.6.1 Biomolecules Coatings

4.6.2 Peptide Grafting

4.7 Conclusion

Acknowledgements

5 Influence of Controlled Micro- and Nanoengineered Environments on Stem Cell Fate

5.1 Introduction to Engineered Environments for the Control of Stem Cell Differentiation

5.1.1 Stem Cells Niche In Vivo: A Highly Dynamic and Complex Environment

5.1.2 Mimicking the Stem Cells Niche In Vitro: Engineered Biomaterials

5.2 Mechanoregulation of Stem Cell Fate

5.2.1 From In Vivo to In Vitro: Influence of the Mechanical Environment on Stem Cell Fate

5.2.2 Regulation of Stem Cell Fate by Surface Roughness

5.2.3 Control of Stem Cell Differentiation by Micro- and Nanotopographic Surfaces

5.2.4 Physical Gradients for Regulating Stem Cell Fate

5.3 Controlled Surface Immobilization of Biochemical Stimuli for Stem Cell Differentiation

5.3.1 Micro- and Nanopatterned Surfaces: Effect of Geometrical Constraint and Ligand Presentation at the Nanoscale

5.3.2 Biochemical Gradients for Stem Cell Differentiation

5.4 Three-dimensional Micro- and Nanoengineered Environments for Stem Cell Differentiation

5.4.1 Three-dimensional Mechanoregulation of Stem Cell Fate

5.4.2 Three-dimensional Biochemical Patterns for Stem Cell Differentiation

5.5 Conclusions and Future Perspectives

6 Recent Advances in Nanostructured Polymeric Surface: Challenges and Frontiers in Stem Cells

6.1 Introduction

6.2 Nanostructured Surface

6.3 Stem Cell

6.4 Stem Cell/Surface Interaction

6.5 Microscopic Techniques Used in Estimating Stem Cell/Surface

6.5.1 Fluorescence Microscopy

6.5.2 Electron Microscopy

6.5.3 Atomic Force Microscopy.

6.5.3.1 Instrument

6.5.3.2 Cell Nanomechanical Motion

6.5.3.3 Mechanical Properties

6.6 Conclusions and Future Perspectives

7 Laser Surface Modification Techniques and Stem Cells Applications

7.1 Introduction

7.2 Fundamental Laser Optics for Surface Structuring

7.2.1 Definitive Facts for Laser Surface Structuring

7.2.1.1 Absorptivity and Reflectivity of the Laser Beam by the Material Surface

7.2.1.2 Effect of the Incoming Laser Light Polarization

7.2.1.3 Operation Mode of the Laser

7.2.1.4 Beam Quality Factor

7.2.1.5 Laser Pulse Energy/Power

7.2.2 Ablation by Laser Pulses

7.2.2.1 Focusing the Laser Beam

7.2.2.2 Ablation Regime

7.3 Methods for Laser Surface Structuring

7.3.1 Physical Surface Modifications by Lasers

7.3.1.1 Direct Structuring

7.3.1.2 Beam Shaping Optics

7.3.1.3 Direct Laser Interference Patterning

7.3.2 Chemical Surface Modification by Lasers

7.3.2.1 Pulsed Laser Deposition

7.3.2.2 Laser Surface Alloying

7.3.2.3 Laser Surface Oxidation and Nitriding

7.4 Stem Cells and Laser-modified Surfaces

7.5 Conclusions

8 Plasma Polymer Deposition: A Versatile Tool for Stem Cell Research

8.1 Introduction

8.2 The Principle and Physics of Plasma Methods for Surface Modification

8.2.1 Plasma Sputtering, Etching an Implantation

8.2.2 Plasma Polymer Deposition

8.3 Surface Properties Influencing Stem Cell Fate

8.3.1 Plasma Methods for Tailored Surface Chemistry

8.3.1.1 Oxygen-rich Surfaces

8.3.1.2 Nitrogen-rich Surfaces

8.3.1.3 Systematic Studies and Copolymers

8.3.2 Plasma for Surface Topography

8.3.3 Plasma for Surface Stiffness

8.3.4 Plasma for Gradient Substrata

8.3.5 Plasma and 3D Scaffolds

8.4 New Trends and Outlook

8.5 Conclusions

References.

9 Three-dimensional Printing Approaches for the Treatment of Critical-sized Bone Defects

9.1 Background

9.1.1 Treatment Approaches for Critical-sized Bone Defects

9.1.2 History of the Application of 3D Printing to Medicine and Biology

9.2 Overview of 3D Printing Technologies

9.2.1 Laser-based Technologies

9.2.1.1 Stereolithography

9.2.1.2 Selective Laser Sintering

9.2.1.3 Selective Laser Melting

9.2.1.4 Electron Beam Melting

9.2.1.5 Two-photon Polymerization

9.2.2 Extrusion-based Technologies

9.2.2.1 Fused Deposition Modeling

9.2.2.2 Material Jetting

9.2.3 Ink-based Technologies

9.2.3.1 Inkjet 3D Printing

9.2.3.2 Aerosol Jet Printing

9.3 Surgical Guides and Models for Bone Reconstruction

9.3.1 Laser-based Surgical Guides

9.3.2 Extrusion-based Surgical Guides

9.3.3 Ink-based Surgical Guides

9.4 Three-dimensionally Printed Implants for Bone Substitution

9.4.1 Laser-based Technologies for Metallic Bone Implants

9.4.2 Extrusion-based Technologies for Bone Implants

9.4.3 Ink-based Technologies for Bone Implants

9.5 Scaffolds for Bone Regeneration

9.5.1 Laser-based Printing for Regenerative Scaffolds

9.5.2 Extrusion-based Printing for Regenerative Scaffolds

9.5.3 Ink-based Printing for Regenerative Scaffolds

9.5.4 Pre- and Post-processing Techniques

9.5.4.1 Pre-processing

9.5.4.2 Post-processing: Sintering

9.5.4.3 Post-processing: Functionalization

9.6 Bioprinting

9.7 Conclusion

List of Abbreviation

10 Application of Bioreactor Concept and Modeling Techniques to Bone Regeneration and Augmentation Treatments

10.1 Bone Tissue Regeneration

10.1.1 Proinflammatory Cytokines

10.1.2 Transforming Growth Factor Beta

10.1.3 Angiogenesis in Regeneration.

10.2 Actual Therapeutic Strategies and Concepts to Obtain an Optimal Bone Quality and Quantity

10.2.1 Guided Bone Regeneration Based on Cells

10.2.1.1 Embryonic Stem Cells

10.2.1.2 Adult Stem Cells

10.2.1.3 Mesenchymal Stem Cells

10.2.2 Guided Bone Regeneration Based on Platelet-Rich Plasma (PRP) and Growth Factors

10.2.2.1 Bone Morphogenetic Proteins

10.2.3 Guided Bone Regeneration Based on Barrier Membranes

10.2.4 Guided Bone Regeneration Based on Scaffolds

10.3 Bioreactors Employed for Tissue Engineering in Guided Bone Regeneration

10.3.1 Spinner Flask Bioreactors

10.3.2 Rotating Wall Bioreactors

10.3.3 Perfusion Bioreactors

10.4 Bioreactor Concept in Guided Bone Regeneration and Tissue Engineering: In Vivo Application

10.4.1 Sand Blasting

10.4.2 Chemical Treatment

10.4.3 Heat Treatment

10.5 New Multidisciplinary Approaches Intended to Improve and Accelerate the Treatment of Injured and/or Diseased Bone

10.5.1 Application of Bioreactor in Dentistry: Therapies for the Treatment of Maxillary Bone Defects

10.5.2 Application of Bioreactor in Cases of Osteoporosis

10.6 Computational Modeling: An Effective Tool to Predict Bone Ingrowth

11 Stem Cell-based Medicinal Products: Regulatory Perspectives

11.1 Introduction

11.2 Defining Stem Cell-based Medicinal Products

11.3 Regional Regulatory Issues for Stem Cell Products

11.4 Regulatory Systems for Stem Cell-based Technologies

11.4.1 The US Regulatory System

11.5 Stem Cell Technologies: The European Regulatory System

12 Substrates and Surfaces for Control of Pluripotent Stem Cell Fate and Function

12.1 Introduction

12.2 Pluripotent Stem Cells

12.3 Substrates for Maintenance of Self-renewal and Pluripotency of PSCs

12.3.1 Cellular Substrates

12.3.2 Acellular Substrates.

12.3.2.1 Biological Matrices.

Notes:

Includes index.

Includes bibliographical references at the end of each chapters and index.

Description based on print version record.

ISBN:

9781119242826

1119242827

9781119242642

1119242649

OCLC:

959698552