Identification of the 'active' fraction and metabolic pathways in trace organic contaminants removal using stable isotope probing / by Kartik Chandran, Ph.D. Columbia University.

Format:

Book

Author/Creator:

Chandran, Kartik, author.

Series:

WERF Research Report Series

WERF Research Report Series ; v.U2R12

Language:

English

Subjects (All):

Environmental sciences.

Economic development--Environmental aspects.

Economic development.

Physical Description:

1 online resource (74 pages) : illustrations, tables

Edition:

1st ed.

Place of Publication:

Alexandria, Virginia : WERF, 2016.

Summary:

The widespread presence of trace organic contaminants (TOrC), such as the endocrine disrupting compound bisphenol-A (BPA), has been cause for growing concern due to persistence in the environment and potential ecological impacts.

Contents:

Cover

Copyright

Acknowledgments

Abstract and Benefits

Table of Contents

List of Tables

List of Figures

List of Acronyms

Executive Summary

Chapter 1.0: Introduction

1.1 Background

1.2 Biodegradation of Trace Organic Compounds in Activated Sludge

1.3 Bisphenol-A as Trace Organic Compound of Interest

1.4 Tools to Elucidate the 'Active' Fraction and Metabolic Pathways of TOrC Biodegradation in Activated Sludge

1.5 Organization of Report

Chapter 2.0: DNA-SIP Protocol Optimization

2.1 Stable Isotope Probing Overview

2.2 Establishment of Optimal Experimental Conditions for DNA-SIP Batch Experiments

2.3 Optimization of Separation by Density Gradient Ultracentrifugation

2.4 Evaluation of DNA Visualization Techniques

Chapter 3.0: Techniques and Methods for BPA Degradation Studies and DNA-SIP Batch Experiments

3.1 Mixed Culture Nitrifying Community

3.1.1 Nitrifying Sequencing Batch Reactor

3.1.2 Evaluation of BPA Biodegradation by Mixed Culture Nitrifying Community

3.2 Source of Biomass for DNA-SIP Experiments

3.3 Exposure of Biomass to BPA

3.3.1 Preliminary Evaluation of BPA Biodegradation

3.3.2 BPA Exposure Conditions

3.3.3 Examination of Microbial Community Structure Changes Due to BPA Exposure Using Next Generation Sequencing

3.3.4 RNA-seq of Microbial Community Exposed to BPA

3.4 DNA-SIP Experiments

3.4.1 DNA Extraction and Density Gradient Ultracentrifugation

3.4.2 Quantification of Gradient Fractions

3.4.3 Characterization of Gradient Fractions Using Next Generation Sequencing

3.5 Analytical Methods

Chapter 4.0: Evaluation of BPA Biodegradation by a Mixed Culture Nitrifying Community

4.1 Nitrifying Sequencing Batch Reactor

4.2 BPA Biodegradation

4.2.1 BPA Biodegradation Experiments: 35 μg/L BPA Spike.

4.2.2 BPA Biodegradation Experiments: 900 μg/L BPA Spike

4.3 Insights Applied to DNA-SIP Experiment Development

Chapter 5.0: BPA Transformation by Microbial Communities from a Full-Scale WWTP

5.1 Evaluation of BPA Biodegradation

5.2 BPA Degradation during BPA Exposure

5.3 Microbial Community Structure Before and After Exposure to BPA

5.4 Metatranscriptomic Analysis of Microbial Community Exposed to BPA

Chapter 6.0: DNA-SIP Using 13C BPA for Microbial Communities from a Full-Scale WWTP

6.1 BPA Biodegradation in SIP Experiments

6.2 Analysis of SIP Gradient Fractions

6.3 Identification of BPA Assimilating Microbes

Chapter 7.0: Conclusions

Appendix A: BPA Biodegradation Metabolite: Time Course Data from DNA-SIP Experiments

References.

Notes:

Includes bibliographical references.

Description based on online resource; title from PDF title page (ebrary, viewed November 24, 2016).

Description based on publisher supplied metadata and other sources.

ISBN:

1-78040-828-5

OCLC:

969637375