Mass spectrometry and stable isotopes in nutritional and pediatric research / edited by Henk Schierbeek.

Format:

Book

Contributor:

Schierbeek, Henk, 1956- editor.

Series:

Wiley-Interscience series on mass spectrometry.

Wiley series on mass spectrometry

Language:

English

Subjects (All):

Biomolecules--Analysis.

Biomolecules.

Mass spectrometry.

Nutrition--Research.

Nutrition.

Pediatrics--Research.

Pediatrics.

Physical Description:

1 online resource (432 pages) : illustrations (some color).

Edition:

1st ed.

Place of Publication:

Hoboken, New Jersey : John Wiley & Sons, Incorporated, [2017]

Summary:

A guide for scientists, pediatricians and students involved in metabolic studies in pediatric research * Addresses the availability of modern analytical techniques and how to apply these techniques in metabolic studies * Covers the whole range of available mass spectrometric techniques used for metabolic studies including Stable Isotope Methodology * Presents the relevance of mass spectrometry and stable isotope methodology in pediatric research covering applications in Nutrition, Obesity, Metabolic Disorders, and Kidney Disorders * Focuses on the interactions between nutrients and the endogenous metabolism within the body and how these factors affect the health of a growing infant

Contents:

Intro

Wiley Series on Mass Spectrometry

Title Page

Copyright

Table of Contents

List of Contributors

Introduction

List of Abbreviations

Chapter 1: Mass Spectrometry Techniques for In Vivo Stable Isotope Approaches

1.1 Introduction

1.2 Nomenclature for Light-Stable Isotope Changes

1.3 Mass Spectrometry Techniques

1.4 Choice of Mass Spectrometric Techniques and Applications to Measure Isotopic Enrichments in Metabolic Studies

1.5 Conclusion and Future Perspectives

References

Chapter 2: Stable Isotope Technology

2.1 History

2.2 Definition

2.3 Safety

2.4 Stable Isotopes and Natural Abundances

2.5 Stable Isotope Selection

2.6 Single or Multiple Label Selection

2.7 Precursor Model

2.8 Simultaneous Infusion

2.9 Infusion Techniques

2.10 Steady State

2.11 Pool Selection

2.12 Pool Models

2.13 Flux: Synthesis and Breakdown

2.14 Nitrogen Balance

2.15 Doubly Labeled Water Method

2.16 Whole-body Protein Synthesis

2.17 Specific Protein Synthesis

2.18 Calculations

2.19 Considerations and Drawbacks of Isotopic Tracers

2.20 Conclusion

Chapter 3: Stable Isotopes in Nutritional and Pediatric Research

3.1 Introduction

3.2 Ethical Aspects

3.3 Applications of Stable Isotopes in Nutritional and Pediatric Research

3.4 Conclusion

Chapter 4: Early-Life Nutrition and Stable Isotope Techniques

4.1 Introduction

4.2 Breast Milk versus Infant Formula

4.3 Techniques to Monitor Milk Intake

4.4 Body Composition in Term and Preterm Infants

4.5 Amino Acid Requirement

4.6 Clinical Applications

4.7 Additional Applications

4.8 Discussion

4.9 Conclusion

4.10 Future Perspectives

Chapter 5: Assessment of Amino Acid Requirement in Children Using Stable Isotopes

5.1 Introduction.

5.2 Nutrient Needs and Definitions

5.3 Methods to Determine Requirements

5.4 Isotopic Tracer Methods

5.5 Existing Methods to Determine Amino Acid Requirement for Neonates

5.6 Use of the IAAO Method in the Pediatric Population

5.7 Necessity for Performing the Study

5.8 Biochemistry

5.11 Analysis and Calculations

5.12 Results

5.13 Statistical Analysis

5.14 Discussion

5.15 Conclusion

5.16 Future Perspectives

Chapter 6: Metabolism of Glutamine, Citrulline, and Arginine

Stable Isotopes Analyzing the Intestinal-Renal Axis

6.1 Introduction

6.2 Biochemistry

6.3 Isotopic Model

6.4 Study Design

6.5 Mass Spectrometry Methods

6.6 Clinical Applications

6.7 Calculations

6.8 Discussion and Future Perspectives

Chapter 7: Applications in Fat Absorption and Metabolism

7.1 Introduction

7.2 Biochemistry of Fat Absorption

7.3 Isotope Model

7.4 Study Design/Infusion Protocols

7.5 Analytical Equipment

7.6 Analytical Conditions

7.7 Accuracy and Precision

7.8 Calculations

7.9 Clinical Applications

7.10 Future Perspectives

Chapter 8: Materno-Fetal Lipid Kinetics

8.1 Introduction

8.2 Biochemistry of Placental Lipid Transport

8.3 Investigation of Fatty Acid Metabolism Using Stable Isotopes

8.4 Mass Spectrometry Methods

8.5 Clinical Studies with Fatty Acids Labeled with Stable Isotopes in Healthy and Complicated Pregnancies

8.6 Calculations

8.7 Future Perspectives

Acknowledgments

Chapter 9: Stable Isotope Applications in Human In Vivo Placental and Fetal Research

9.1 Introduction

9.2 Investigation of Fetal Metabolism Using Stable Isotopes

9.3 Study Designs and Models

9.4 Infusion Protocols and Clinical Applications

9.5 Necessary Additional Clinical Parameters to be Analyzed.

9.6 Necessary Analytical Mass-Spectrometry Equipment and Analytical Conditions

9.7 Calculations

9.8 Future Perspectives

Chapter 10: Obesity

10.1 Introduction

10.2 Singly and Doubly Labeled Water

10.3 Substrate Oxidation

10.4 Glucose Metabolism

10.5 Fat Metabolism

10.6 Protein Turnover

10.7 Calculations

10.8 Discussion and Future Perspectives

Chapter 11: Inborn Errors of Metabolism

11.1 Introduction

11.2 Stable Isotope Techniques

11.3 Analytical Equipment and Methods

11.4 Study Protocol: Quantifying Endogenous Galactose Production

11.5 Calculations

11.6 Discussion

11.7 Future Perspectives

Chapter 12: Renal Disease and Dialysis

12.1 Introduction

12.2 Total Body Water and Its Distribution

12.3 Protein Metabolism in Chronic Kidney Disease

12.4 Dialysis - Metabolic Consequences and Nutrient Losses

12.5 Primary Hyperoxalurias

12.6 Clinical Applications

12.7 Calculations

12.8 Discussion

12.9 Future Perspectives

Chapter 13: Application in Oxidative Stress and Glutathione Metabolism in Preterm Infants

13.1 Introduction

13.2 Biochemistry/Model

13.3 Guidelines and Safety Procedures

13.4 Mass Spectrometry Methods

13.5 Materials and Methods

13.6 Clinical Application (A Practical Example of a Study Protocol)

13.7 Calculations

13.8 Discussion and Future Perspectives

Chapter 14: Nutrient Digestion and Absorption During Intestinal Malfunction and Diseases

14.1 Introduction

14.2 Clinical Application [49]

Index

End User License Agreement.

Notes:

Includes bibliographical references and index.

Description based on print version record.

ISBN:

9781119341185

1119341183

9781119341123

1119341124

9781119341246

1119341248

OCLC:

970042113