The use of mass spectrometry technology (MALDI-TOF) in clinical microbiology / edited by Fernando Cobo.

Format:

Book

Contributor:

Cobo Martínez, Fernando, editor.

Language:

English

Subjects (All):

Mass spectrometry.

Diagnostic microbiology.

Genre:

Electronic books.

Physical Description:

1 online resource

Place of Publication:

London : Academic Press, 2018.

System Details:

text file

Summary:

The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology presents the state-of the-art for MALDI-TOF mass spectrometry. It is a key reference defining how MALDI-TOF mass spectrometry is used in clinical settings as a diagnostic tool of microbial identification and characterization that is based on the detection of a mass of molecules. The book provides updated applications of MALDI-TOF techniques in clinical microbiology, presenting the latest information available on a technology that is now used for rapid microbial identification at relatively low cost, thus offering an alternative to conventional laboratory diagnosis and proteomic identification systems. Although the main use of the technology has, until now, been identification or typing of bacteria from a positive culture, applications in the field of virology, mycology, microbacteriology and resistances are opening up new opportunities.

Contents:

Front Cover; The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology; Copyright Page; Contents; List of Contributors; Preface; 1. Proteomics: Technology and Applications; 1.1 Introduction; 1.2 Definition and Importance of the Proteomics; 1.3 Technical Methods in Proteomics; 1.3.1 Isolation and separation of proteins; 1.3.2 Analysis of the structure of the separated proteins; 1.3.3 Utilization of computer databases to identify the characterized proteins; 1.4 Proteomics' Role in the Clinical Laboratory; 1.5 Clinical Applications of Proteomics; 1.5.1 Microbiology

1.5.2 Biomarkers1.5.3 Immunoproteomics; 1.5.4 Vaccines and drugs; 1.6 Bioinformatics' Application to Proteomics; 1.7 Challenges of Proteomics; 1.8 Conclusions; References; 2. Basis of Mass Spectrometry: Technical Variants; 2.1 Overview; 2.2 MS: Definition and Basic Principles; 2.3 A Brief Excursion to the History of MS and Related Nobel Prizes; 2.4 Basic Components of a Mass Spectrometer; 2.5 Ionization Techniques in Biological MS; 2.6 Mass Analyzers; 2.7 Tandem MS; 2.8 Separation Techniques Hyphenated With MS; 2.9 MSI and Other Ways of Examining Biological Tissues

2.10 Mass Spectrum, Data Representation, and ManagementAcknowledgments; References; 3. MALDI-TOF Commercial Platforms for Bacterial Identification; 3.1 Introduction; 3.2 Commercial MALDI-TOF MS Systems; 3.3 MALDI Biotyper System; 3.4 VITEK MS System; 3.5 MALDI Biotyper vs VITEK MS Systems' Comparison; 3.6 Andromas; References; 4. Work Procedures in MALDI-TOF Technology; 4.1 Introduction; 4.2 Reagents and Equipment; 4.2.1 VITEK MS; 4.2.1.1 Reagents; 4.2.1.2 Supplies; 4.2.1.3 Equipment; 4.2.2 MALDI Biotyper system; 4.2.2.1 Reagents; 4.2.2.2 Supplies; 4.2.2.3 Equipment

4.3 Calibration and Quality Control4.3.1 VITEK MS; 4.3.1.1 Calibration; 4.3.1.2 Controls; 4.3.2 MALDI Biotyper CA system; 4.3.2.1 Calibration; 4.3.2.2 Controls; 4.4 Preanalytical Processing Methods for Protein Extraction; 4.4.1 Specimen collection; 4.4.2 MALDI Biotyper clinical application system extraction protocol; 4.4.3 VITEK MS extraction protocol; 4.4.4 Special sample preparation methods (research use only); 4.4.4.1 Aerobic actinomycetes; 4.4.4.1.1 Nocardia; 4.4.4.1.2 Mycobacteria; 4.4.4.2 Filamentous fungi; 4.5 Analytical Procedure; 4.5.1 MALDI Biotyper system; 4.5.1.1 Inoculation

4.5.1.2 Spectrum acquisition4.5.1.3 Identification; 4.5.2 VITEK MS; 4.5.2.1 Inoculation; 4.5.2.2 Spectrum acquisition; 4.5.2.3 Identification; 4.6 Cleaning MALDI-TOF MS Target Plates; References; 5. Indications, Interpretation of Results, Advantages, Disadvantages, and Limitations of MALDI-TOF; 5.1 Introduction; 5.2 Indications for the Use of MALDI-TOF in Clinical Microbiology; 5.3 Interpretation of Results; 5.4 Advantages and Disadvantages; 5.4.1 Advantages; 5.4.2 Disadvantages; 5.5 Limitations of MALDI-TOF Technique; 5.5.1 Typing; 5.5.2 Resistance and virulence factors

Notes:

Online resource; title from PDF title page (EBSCO, viewed August 9, 2018)

ISBN:

9780128144527

0128144521

OCLC:

1048003758

Access Restriction:

Restricted for use by site license.