Mucins : Methods and Protocols / edited by Michael A. McGuckin, David J. Thornton.

Format:

Book

Contributor:

McGuckin, Michael A., editor.

Thornton, David J., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 842.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 842

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Proteomics.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Proteomics.

Physical Description:

1 online resource (XI, 329 pages) : 48 illustrations, 4 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucins. These include methods and techniques for the best approaches to analysing each specific area of mucin biochemistry, physiology and biophysics before providing individual detailed experimental protocols together with troubleshooting and interpretation tips. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mucins: Methods and Protocols is designed to be a useful resource for those entering the mucin field and to facilitate those already studying mucins to broaden their experimental approaches to understanding mucosal biology.

Contents:

Mucin Methods: Genes Encoding Mucins And Their Genetic Variation With A Focus On Gel Forming Mucins

Gel-forming and Cell-associated Mucins - preparation for Structural and Functional Studies

Detecting, Visualising and Quantifying Mucins

Mass Spectrometric Analysis of Mucin Core Proteins

O-Glycoprotein Biosynthesis: Site Localization by Edman Degradation and Site Prediction Based on Random Peptide Substrates

Analysis of Assembly of Secreted Mucins

MUC1 Membrane Trafficking: Protocols for Assessing Biosynthetic Delivery, Endocytosis, Recycling and Release Through Exosomes

Glycomic Work-flow for Analysis of Mucin O-linked Oligosaccharides

O-Glycomics: Profiling and Structural Analysis of Mucin-type O-linked Glycans

O-Glycoproteomics: Site-specific O-glycoprotein Analysis by CID/ETD Electrospray Ionization Tandem Mass Spectrometry and Top-Down Glycoprotein Sequencing by In-Source Decay MALDI Mass Spectrometry

Analysing Mucin Degradation

Assessment of Mucus Thickness and Production In Situ

Preservation of Mucus in Histological Sections, Immunostaining of Mucins in Fixed Tissue and Localization of Bacteria with FISH

In vitro Measurements of Mucus Secretion by Colon Explants

Establishment of Respiratory Air-Liquid Interface Cultures and their Use in Studying Mucin Production, Secretion, and Function

Studying Mucin Secretion from Human Bronchial Epithelial Cell Primary Cultures

Assessment of Intracellular Mucin Content In Vivo

Techniques for Assessment of Interactions of Mucins with Microbes and Parasites In Vitro and In Vivo

Assessing Mucin Expression and Function in Human Ocular Surface Epithelia In Vivo and In Vitro.

Other Format:

Printed edition:

ISBN:

9781617795138

Access Restriction:

Restricted for use by site license.