Plant Transcription Factors : Methods and Protocols / edited by Nobutoshi Yamaguchi.

Format:

Book

Contributor:

Yamaguchi, Nobutoshi, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1830.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1830

Language:

English

Subjects (All):

Life sciences.

Botany.

Life Sciences.

Plant Sciences.

Local Subjects:

Life Sciences.

Plant Sciences.

Physical Description:

1 online resource (XII, 396 pages) : 81 illustrations, 58 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.

Contents:

Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective

Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants

The Long-Term 'In Natura' Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments

Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs

A Specific Knockdown of Transcription Factor Activities in Arabidopsis

Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis

CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha

Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines

Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells

Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation

High Impact Gene Discovery: Simple Strand Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network

Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti

nanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing

Genome-Wide TSS Identification in Maize

Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee

Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci

Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines

Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA Binding Interactions and Identify cis-Elements

In Situ Proximity Ligation Assay to Detect the Interaction between Plant Transcription Factors and Other Regulatory Proteins

Cell-Free Protein Synthesis of Plant Transcription Factors

Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants

Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato

Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants.

Other Format:

Printed edition:

ISBN:

9781493986576

Access Restriction:

Restricted for use by site license.