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Metalloproteins : Methods and Protocols / edited by Yilin Hu.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 1876.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 1876
- Language:
- English
- Subjects (All):
- Biochemistry.
- Protein Science.
- Local Subjects:
- Protein Science.
- Physical Description:
- 1 online resource (X, 275 pages) : 62 illustrations, 40 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- New York, NY : Springer New York : Imprint: Humana Press, 2019.
- System Details:
- text file PDF
- Summary:
- This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.
- Contents:
- Nitrogenases
- Bacteriochlorophyll and Coenzyme F430
- Carbon Monoxide Dehydrogenases
- Molybdenum-Containing Enzymes
- Hydrogenases
- Genomic Manipulations of the Diazotroph Azotobacter vinelandii
- Purification of Nitrogenase Proteins
- Expression, Purification and Activity Analysis of Chlorophyllide Oxidoreductase and Ni2+-Sirohydrochlorin a,c-Diamide Reductase
- Reconstitution of Molybdoenzymes with Bis-Molybdopterin Guanine Dinucleotide Cofactor
- Crystallization of Nitrogenase Proteins
- X-Ray Crystallography of Carbon Monoxide Dehydrogenases
- X-Ray Absorption Spectroscopy of Metalloproteins
- Electron Paramagnetic Resonance Spectroscopy of Metalloproteins
- Magnetic Circular Dichroism Spectroscopy of Metalloproteins
- Chemical Synthesis of an Asymmetric Mimic of the Nitrogenase Active Site
- Computational Methods for Modeling Metalloproteins.
- Other Format:
- Printed edition:
- ISBN:
- 9781493988648
- Access Restriction:
- Restricted for use by site license.
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