Lymphoma : Methods and Protocols / edited by Tim Illidge, Peter W. M. Johnson.

Format:

Book

Contributor:

Illidge, Timothy, editor.

Johnson, P. W. M., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 115.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 115

Language:

English

Subjects (All):

Medicine.

Cancer--Research.

Cancer.

Biomedicine.

Cancer Research.

Local Subjects:

Biomedicine.

Cancer Research.

Physical Description:

1 online resource (XVIII, 342 pages) : 43 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

The tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas.

Contents:

Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins

Molecular Diagnosis of Lymphoma

Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cytometry

Karyotyping Lymph Node Biopsies in Non-Hodgkin's Lymphoma

Identification of Lymphoma-Associated Antigens Using SEREX

Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia

Idiotype Gene Rescue in Follicular Lymphoma

Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma

T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma

Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain Reaction

Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic Leukemia

Splenic Marginal Zone Lymphoma

BCL-6

Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL

Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5?RACE

Follicular Lymphoma.

Other Format:

Printed edition:

ISBN:

9781592599363

Access Restriction:

Restricted for use by site license.