Therapeutic Applications of Ribozymes and Riboswitches : Methods and Protocols / edited by Daniel Lafontaine, Audrey Dubé.

Format:

Book

Contributor:

Lafontaine, Daniel, editor.

Dubé, Audrey (Biologist), editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1103.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1103

Language:

English

Subjects (All):

Medicine.

Human genetics.

Pharmacology.

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Physical Description:

1 online resource (XI, 278 pages) : 53 illustrations, 19 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.

System Details:

text file PDF

Summary:

In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.

Contents:

Identification of Regulatory RNA in Bacterial Genomes by Genome-Scale Mapping of Transcription Start Sites

Screening Inhibitory potential of anti-HIV RT RNA Aptamers

Design and Evaluation of Clinically Relevant SOFA-HDV Ribozymes Targeting HIV RNA

Directing RNase P-mediated Cleavage of Target mRNAs by Engineered External Guide Sequences in Cultured Cells

Design and Analysis of Hammerhead Ribozyme Activity Against an Artificial Gene Target

Knockdown Strategies for the Study of Proprotein Converstase and Proliferation in Prostate Cancer Cells

Use of Tumor-Targeting Trans-Splicing Ribozyme for Cancer Treatment

Characterization of Hairpin Ribozyme Reactions

Finding Instances of Riboswitches and Ribozymes by Homology Search of Structured RNA with Infernal

Structure-based Virtual Screening for the Identification of RNA-binding Ligands

Probing Riboswitch Binding Sites with Molecular Docking, Focused Libraries, and in-line Probing Assays

Discovery of Small Molecule Modifiers of microRNAs for the Treatment of HCV Infection

Bacterial Flavin Mononucleotide Riboswitches as Targets for Flavin Analogs

Construction and Application of Riboswitch-based Sensors That Detect Metabolites Within Bacterial Cells

Screening Assays to Identify Artificial glmS Ribozyme Activators

Analysis of Riboswitch Structure and Ligand Binding using Small-angle X-ray Scattering (SAXS)

Use of SHAPE to Select 2AP Substitution sites for RNA-ligand Interactions and Dynamics Studies

Cell Internalization SELEX: In Vitro Selection for Molecules that Internalize into Cells

DNA Electronic Switches Based on Analyte-Responsive Aptamers.

Other Format:

Printed edition:

ISBN:

9781627037303

Access Restriction:

Restricted for use by site license.