Nuclear G-Protein Coupled Receptors : Methods and Protocols / edited by Bruce G. Allen, Terence E. Hébert.

Format:

Book

Contributor:

Allen, Bruce G., editor.

Hébert, Terence E., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1234.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1234

Language:

English

Subjects (All):

Life sciences.

Proteins.

Life Sciences.

Protein Science.

Local Subjects:

Life Sciences.

Protein Science.

Physical Description:

1 online resource (XV, 215 pages) : 43 illustrations, 15 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

Nuclear G-Protein Coupled Receptors: Methods and Protocols is a compilation of a number of conceptual and methodological aspects important for the validation and characterization of intacrine signaling systems. To date, the best-characterized intracrine signaling system is that of angiotensin II (Ang II), covered in depth in various chapters. Methodology to study the subcellular localization and function of GPCRs and other signaling systems is provided, as well as numerous chapters focusing on methods designed to understand signaling mediated by nuclear and other internal GPCRs. Methods are also described to study the formation of second messengers such as cAMP and to study the trafficking of receptors from the cell surface. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nuclear G-Protein Coupled Receptors: Methods and Protocols seeks to serve both professionals and novices with state-of-the-art approaches to characterize what is becoming a common theme in cellular signaling.

Contents:

Studies of Intracellular Angiotensin II

Single-Cell Microinjection Coupled to Confocal Microscopy to Characterize Nuclear Membrane Receptors in Freshly Isolated Cardiomyocytes

Design and Application of Light-Activated Probes for Cellular Signaling

Using Caged Ligands to Study Intracrine Endothelin Signalling In Intact Cardiac Myocytes

Quantification of Catecholamine Uptake in Adult Cardiac Myocytes

Characterization of the Interaction Between the Prostaglandin D2 DP1 Receptor and the Intracellular L-Prostaglandin D Synthase

Isolation and Study of Cardiac Nuclei from Canine Myocardium and Adult Ventricular Myocytes

High Resolution Imaging and Function of Nuclear G Protein-Coupled Receptors (GPCRs)

Biochemical Fractionation of Membrane Receptors in the Nucleus

Functional G Protein-Coupled Receptors on Nuclei From Brain and Primary Cultured Neurons

Automated Microscopy of Cardiac Myocyte Hypertrophy: A Case Study on the Role of Intracellular a-Adrenergic Receptors

Measuring Intra-Nuclear and Nuclear Envelope [Ca2+] vs. Cytosolic [Ca2+]

Assessing GPCR and G Protein Signaling to the Nucleus in Live Cells Using Fluorescent Biosensors

Tandem Affinity Purification to Identify Cytosolic and Nuclear Gbg-Interacting Proteins

Examining the Effects of Nuclear GPCRs on Gene Expression Using Isolated Nuclei

Trafficking and Function of GPCRs in the Endosomal Compartment.

Other Format:

Printed edition:

ISBN:

9781493917556

Access Restriction:

Restricted for use by site license.