Single Domain Antibodies : Methods and Protocols / edited by Dirk Saerens, Serge Muyldermans.

Format:

Book

Contributor:

Saerens, Dirk, editor.

Muyldermans, Serge, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 911.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 911

Language:

English

Subjects (All):

Medicine.

Immunology.

Immunoglobulins.

Biomedicine.

Antibodies.

Local Subjects:

Biomedicine.

Immunology.

Antibodies.

Physical Description:

1 online resource (XV, 580 pages) : 89 illustrations, 35 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2012.

System Details:

text file PDF

Summary:

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Contents:

From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

Introduction to Heavy Chain Antibodies and Derived Nanobodies

Overview and Discovery of IgNARs and Generation of VNARs

Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

Preparation of a Naïve Library of Camelid Single Domain Antibodies

Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation

Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens

Pichia Surface Display: A Tool for Screening Single Domain Antibodies

Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method

Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A "One Plasmid" Mammalian Two-Hybrid System

Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization

Selection of VHHs Under Application Conditions

Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies

Selection of VHH Antibody Fragments that Recognize Different Aβ Depositions Using Complex Immune Libraries

Expression of Single-Domain Antibodies in Bacterial Systems

Expression of VHHs in S. cerevisiae

Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells

Production of Camel-Like Antibodies in Plants

Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains

Solubility and Stability Engineering of Human VH Domains

Improvement of Proteolytic Stability through In Silico Engineering

Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display

Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction

Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond

Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display

Multivalent Display of Single-Domain Antibodies

Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology

Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding

Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide

Nanobody-Based Chromatin Immunoprecipitation

User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags

Application of Single Domain Antibodies in Tumor Histochemistry

Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation

Molecular Imaging Using Nanobodies: A Case Study

Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis.

Other Format:

Printed edition:

ISBN:

9781617799686

Access Restriction:

Restricted for use by site license.