Current Laboratory Methods in Neuroscience Research / edited by Huangui Xiong, Howard E. Gendelman.

Format:

Book

Contributor:

Xiong, Huangui, editor.

Gendelman, Howard E., 1954- editor.

SpringerLink (Online service)

Series:

Springer protocols (Series) 1949-2448

Springer Protocols (Springer-12345)

Springer Protocols Handbooks, 1949-2448

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Neurology.

Neurobiology.

Biomedicine.

Local Subjects:

Biomedicine.

Neurosciences.

Neurology.

Neurobiology.

Physical Description:

1 online resource (XIII, 585 pages) : 189 illustrations, 122 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Springer, 2014.

System Details:

text file PDF

Summary:

Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a "blueprint" for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Contents:

Section 1: Neurohistological techniques

1. Brain tissue preparation, sectioning and staining

2. Brain stereotactic injections

3. Tract tracing at light and electron microscopy

4. Stereological analysis of neurological tissues

Section 2: In vitro preparations

5. Preparation and use of rodent hippocampal brain slices

6. Single-Cell Neuronal Dissociation For Electrophysiological Studies

7. Isolation and Culture of Human Neurons, Microglia and Astrocytes

8. Isolation and Culture of Neural Stem/progenitor Cells

9. Isolation of mitochondria from brain tissue and cells

10. Isolation of synaptosomes from archived brain tissues

11. Xenopus oocytes isolation and microinjection

Section 3: Leukocyte isolation and application in neuroscience

12. Centrifugal elutriation for studies of neuroimmunity

13. Flow cytometry in neuroscience research

Section 4: Standard Laboratory Nucleic Acid and Protein Detections

14. Western Blotting Technique in Biomedical Research

15. Polymerase chain reaction (PCR) and Real-time RT PCR

Section 5: Nanomedicine

16. Nanoformulations

17. Neuronanomedicine

Section 6: Bioimaging

18. Confocal imaging of nerve cells

19. Magnetic resonance imaging (MRI)

20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging

21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography

22. Multiphoton Brain Imaging

Section 7: Neuroelectrophysiology

23. Techniques for Extracellular Recordings

24. "Blind" patch-clamp recordings from rodent hippocampal slices

25. Whole-cell patch clamp recordings

Section 8: Immunohistochemistry and autoradiography

26. Analysis of Receptor Binding And Quantitative Autoradiography

27. Techniques in Immunohistochemistry and immunocytochemistry

Section 9: Analysis of Gene Expression

28. Protein identification by mass spectrometry: Proteomics

29. DNA Microarrays as a Tool for Neurosciences Research

30. Metabolomics

31. Bioinformatic Methods and Resources for Neuroscience Research

Section 10: Animal Models: Behavior and Pathology

32. Motor function in rodent models of neurodegenerative disorders

33. Humanized mice

34. Animal models for PD and ALS

35. Animal models for Alzheimer's Disease

36. Animal Models: Behavior and Pathology.

Other Format:

Printed edition:

ISBN:

9781461487944

Access Restriction:

Restricted for use by site license.