Cell Culture Techniques / edited by Michael Aschner, Cristina Suñol, Anna Bal-Price.

Format:

Book

Contributor:

Aschner, Michael, 1955- editor.

Suñol, Cristina, editor.

Bal-Price, Anna, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 56.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 56

Language:

English

Subjects (All):

Neurosciences.

Neurochemistry.

Local Subjects:

Neurosciences.

Neurochemistry.

Physical Description:

1 online resource (XIV, 498 pages) : 84 illustrations.

Edition:

1.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory. Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.

Contents:

Guidance on Good Cell Culture Practice (GCCP)

Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology

Neural Stem Cells

Primary Cultures for Neurotoxicity Testing

Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening

Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans

Modeling the Blood-Brain Barrier

In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research

Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies

P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions

Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems?

Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy

The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity

Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture

Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death

Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures

Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation

Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology

Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity

13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells

Culture Models for the Study of Amino Acid Transport and Metabolism

Neurotransmitter Transporters and Anticonvulsant Drug Development

Ion Channel Electrophysiology in Cultured Neurons

Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips

GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing.

Other Format:

Printed edition:

ISBN:

9781617790775

Access Restriction:

Restricted for use by site license.