Pancreatic Cancer : Methods and Protocols / edited by Gloria H. Su.

Format:

Book

Contributor:

Su, Gloria H., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 103.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 103

Language:

English

Subjects (All):

Medicine.

Oncology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Oncology.

Physical Description:

1 online resource (XI, 352 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

In the last decade, major progress has been made in identifying the critical oncogenes and tumor-suppressor genes that can cause pancreatic cancer. In Pancreatic Cancer: Methods and Protocols, leading clinicians and scientists from prestigious research institutes and cancer centers present in readily reproducible format the classical techniques that have contributed to these advances in pancreatic research and introduce new strategies that should produce future breakthroughs across the field of cancer biology. The authors provide a broad range of methods for the molecular, biochemical, pathological, and statistical analyses of both sporadic and familial pancreatic cancers, methods that can be applied not only to basic, but also to translational pancreatic research. Topics covered include in vitro cell cultures, in vivo mouse models, protein studies, mutation analysis, and treatment development. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pancreatic Cancer: Methods and Protocols offers those interested in either basic or translational research an ambitious collection of essential yet novel methods for pancreatic cancer research, methods that can also readily be applied broadly throughout cancer research.

Contents:

Identification and Analysis of Precursors to Invasive Pancreatic Cancer

Optimal Molecular Profiling of Tissue and Tissue Components

Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia

Practical Methods for Tissue Microarray Construction

Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis

Culture and Immortalization of Pancreatic Ductal Epithelial Cells

DNA Methylation Analysis in Human Cancer

Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance

Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes

Serial Analyses of Gene Expression (SAGE)

Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma

Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach

Detection of Telomerase Activity in Patients with Pancreatic Cancer

Serological Analysis of Expression cDNA Libraries (SEREX)

Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses

Strategies for the Use of Site-Specific Recombinases in Genome Engineering

Primary Explant Cultures of Adult and Embryonic Pancreas

Zebrafish as a Model for Pancreatic Cancer Research

Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine

Overview of Linkage Analysis.

Other Format:

Printed edition:

ISBN:

9781592597802

Access Restriction:

Restricted for use by site license.