Toll-Like Receptors : Methods and Protocols / edited by Claire E. McCoy, Luke A. J. O'Neill.

Format:

Book

Contributor:

McCoy, Claire E., editor.

O'Neill, Luke A. J., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 517.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 517

Language:

English

Subjects (All):

Medicine.

Immunology.

Cytokines.

Growth factors.

Biomedicine.

Cytokines and Growth Factors.

Local Subjects:

Biomedicine.

Immunology.

Cytokines and Growth Factors.

Physical Description:

1 online resource (XXIV, 376 pages) : 71 illustrations, 5 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.

System Details:

text file PDF

Summary:

In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls. Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.

Contents:

Methods to Detect and Analyze Toll-Like Receptors

Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells

Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action

Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution

Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding

Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures

Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains

Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation

Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology

2D-DIGE: Comparative Proteomics of Cellular Signalling Pathways

MAPPIT (Mammalian Protein-Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling

Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation

Analysis of Ubiquitin Degradation and Phosphorylation of Proteins

The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands

Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation

Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction

Genetic Techniques in Toll-Like Receptor Analysis

Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis

Microarray Experiments to Uncover Toll-Like Receptor Function

Uncovering Novel Gene Function in Toll-Like Receptor Signalling Using siRNA

Genotyping Methods to Analyse Polymorphisms in Toll-Like Receptors and Disease

Toll-Like Receptors and Disease

Experimental Models of Acute Infection and Toll-Like Receptor Driven Septic Shock

Toll-Like Receptors and Rheumatoid Arthritis

Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine

Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells

Innate Immunity, Toll-Like Receptors, and Atherosclerosis: Mouse Models and Methods

Generation of Parasite Antigens for Use in Toll-Like Receptor Research

Biomarkers Measuring the Activity of Toll-Like Receptor Ligands in Clinical Development Programs.

Other Format:

Printed edition:

ISBN:

9781597455411

Access Restriction:

Restricted for use by site license.