Meningococcal Vaccines : Methods and Protocols / edited by Andrew J. Pollard, Martin C.J. Maiden.

Format:

Book

Contributor:

Pollard, Andrew J., editor.

Maiden, Martin C. J., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 66.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 66

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Medicine & Public Health.

Infectious Diseases.

Local Subjects:

Medicine & Public Health.

Infectious Diseases.

Physical Description:

1 online resource (XVII, 421 pages) : 20 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2001.

System Details:

text file PDF

Summary:

The development of a comprehensive vaccine against Neisseria meningitidis, the causative agent of meningococcal disease, has remained elusive because of bacterial diversity and immunologic evasion. In Meningococcal Vaccines: Methods and Protocols, Andrew Pollard and Martin Maiden have assembled an impressive collection of the latest molecular and cellular techniques for the development, evaluation, and implementation of vaccines to be used against this dreaded disease. The contributors-leading scientists, clinicians, and public health physicians-describe in detail the many approaches to vaccine design, as well as the assessment of immune response to vaccine candidates and novel vaccine formulations. They also present as a test case the recent implementation of a new meningococcal vaccine in the United Kingdom. A companion volume, Meningococcal Disease: Methods and Protocols, contains detailed methods for diagnostic microbiology, bacterial characterization, epidemiology, host-pathogen interactions, and clinical studies. Timely and comprehensive, Meningococcal Vaccines: Methods and Protocols provides the scientist, public health physician, epidemiologist, clinical microbiologist, and clinician with the essential tools to lay bare the secrets of the meningococcus and to develop, evaluate, and implement successful new meningococcal vaccines.

Contents:

Meningococcal Vaccines and Vaccine Developments

Immune Response and Host-Pathogen Interactions

Purification of Capsular Polysaccharide

Protein-Polysaccharide Conjugation

N-Propionylation

Outer Membrane Protein Purification

Outer Membrane Protein Vesicle Vaccines for Meningococcal Disease

Methods for Manipulation of Transferrin-Binding Proteins

Methods for the Preparation and Crystallization of Fab Fragments in Complex with Peptide Antigens Derived from N. meningitidis Outer Membrane Proteins

Application of Optical Biosensor Techniques to the Characterization of PorA-Antibody Binding Kinetics

Construction of porA Mutants

Construction of LPS Mutants

Recombinant Proteins in Vaccine Development

Identification of Peptides that Mimic N. meningitidis LOS Epitopes Via the Use of Combinatorial Phage-Display Libraries

Testing Meningococcal Vaccines for Mitogenicity and Superantigenicity

Early Experiences with Nasal Vaccines against Meningococcal Disease

Animal Models for Meningococcal Disease

Determination of Antibody Responses to Meningococcal Antigens by ELISA

Immunoblot Analysis of Sera from Patients and Vaccinees

Serogroup B and C Serum Bactericidal Assays

A Modified ELISA for Measurement of High-Avidity IgG Antibodies to Meningococcal Serogroup C Polysaccharide that Correlate with Bactericidal Titers

Whole-Blood Model

Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry

T-Cell Responses Against Meningococcal Antigens

CD4 T-Cell Epitope Mapping

Epitope Mapping

Meningococcal Vaccine Trials

The Introduction of Group C Conjugate Meningococcal Vaccine into the UK.

Other Format:

Printed edition:

ISBN:

9781592591480

Access Restriction:

Restricted for use by site license.