Hepatitis C Protocols / edited by Johnson Yiu-Nam Lau.

Format:

Book

Contributor:

Lau, Johnson Yiu-Nam, editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 19.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 19

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Medicine & Public Health.

Infectious Diseases.

Local Subjects:

Medicine & Public Health.

Infectious Diseases.

Physical Description:

1 online resource (XXI, 621 pages) : 171 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

In Hepatitis C Protocols, Johnson YN Lau and his expert collaborators present cutting-edge techniques for study of the hepatitis C virus infection and its disease, hepatitis C, which afflict an estimated 170 million people worldwide. The methods range from the detection of anti-HCV in serum and HCV in liver tissue, to HCV genotyping and the detection and quantification of HCV in serum. In addition to these key protocols, the authoritative contributors also evaluate recombinant proteins in immunological test systems, including the identification of important antigenic epitopes, antibodies, and recombinant antigens. Molecular and immunological techniques to study this virus and its infection, as well as animal models and recombinant virus technology commonly used to express hepatitis C viral proteins, are also developed. Hepatitis C Protocols summarizes the state-of-the-art in today's research and understanding of the virology of hepatitis C virus. The methods and systems detailed in this book will play a key role in the development of more effective antivirals, in the development of vaccines, and in a deeper understanding of viral replication strategies, viron structure, and the mechanisms of viron assembly and release. A valuable new standard resource for all researchers and clinicians, Hepatitis C Protocols leads the way toward improved prevention, diagnosis, and treatment of viral hepatitis C.

Contents:

Detection of Anti-HCV in Serum

Detection of Anti-GOR Antibodies in HCV Infection

Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III

Detection and Quantitation of HCV in Serum

Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization

Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR)

The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA

Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology

Quality Control of the Polymerase Chain Reaction

Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays

Detection of HCV in Liver Tissue

Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR

Quantification of HCV RNA in Liver Tissue by bDNA Assay

HCV Genotypes

Hepatitis C Virus

Molecular Evolutionary Analysis

Genotyping by Type-Specific Primers That Can Type HCV Types 1-6

Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region

Determination of HCV Genotypes by RFLP

HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II

Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region

Determination of HCV Quasispecies by Cloning and Sequencing

Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism

Hepatitis C Virus Heteroduplex Tracking Assay

Detection of HCV In Situ

In Situ Detection of HCV

In Situ Detection of Hepatitis C Viral Antigens

In Situ Hybridization and the Detection and Localization of HCV RNA

The In Situ Detection of PCR-Amplified Hepatitis C RNA

lmmunoelectron Microscopic Characterization of HCV

Molecular Biologic Characterization

Cloning and Assembly of Complex Libraries of Full-Length HCV cDNA Clones

Use of the Vaccinia Virus/T7 Expression System for Studying HCV Protein Processing

Use of a Discistronic Vector for the Quantitation of HCV IRES Activity

Expression and Dimerization of Hepatitis C Virus Core Protein in E. coli

Expression and Characterization of the HCV NS2 Protease

Expression and Characterization of HCV NS3 Protease

Expression and Characterization of the HCV NS3 Helicase Domain

Hepatitis C Virus RNA-Dependent RNA Polymerase (NS5B Polymerase)

Detection and Molecular Cloning of the Extreme 3?-End of HCV

In Vitro Stability of Hepatitis C Virus RNA

Methods for the Study of Sequence-Specific Binding of Proteins to the HCV RNA Genome

HCV-Specific Immunologic Response

Role of Immune Response in HCV

Determination of Hepatitis C Virus-Specific CD4+ T-Cell Activity in PBMC

Measurement of HCV-Specific CD8+ Cytotoxic T-Cell Activities in the Peripheral Blood by Europium Release Assay

Determination of HCV-Specific Bulk CD8+ Activity in Liver

Characterization of HCV-Specific Cytotoxic T-Lymphocytes from Liver Tissue

Production of Human Monoclonal Antibodies to Hepatitis C Virus and Their Characterization

In Vitro Culture Model

Specific Detection of Negative Strand RNA of Hepatitis C Virus Using Chemical RNA Modification

Strand-Specific rTth RT-PCR for the Analysis of HCV Replication

Cell Culture Systems for the Detection of HCV Infection

Replication and Detection of Hepatitis C Virus in Liver-Derived Cell Lines

Primary Human Hepatocyte Culture for the Study of HCV

A Cultivation Method for Highly Differentiated Primary Chimpanzee Hepatocytes Permissive for Hepatitis C Virus Replication

Construction of Recombinant Viral Vectors for the Expression of HCV Genes

Construction of Recombinant Vaccinia Virus Expressing HCV Genes

Construction of Recombinant Adeno-Associated Virus (AAV)

Production of Replication-Deficient Adenovirus Recombinants

Generation of Recombinant Herpes Simplex Virus Ampiicons

Construction of Recombinant Sindbis-Based Expression Vectors for the Study of HCV Genes and Their Products

In Vivo Models

The Chimpanzee Model

Liver Transplantation as a Model to Study Hepatitis C Virus Infection.

Other Format:

Printed edition:

ISBN:

9781592592609

Access Restriction:

Restricted for use by site license.