Microbial Steroids : Methods and Protocols / edited by José-Luis Barredo, Ignacio Herráiz.

Format:

Book

Contributor:

Barredo, José-Luis, editor.

Herráiz, Ignacio, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1645.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1645

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (XI, 373 pages) : 47 illustrations, 27 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.

Contents:

Steroid Bioconversions

Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins

Genome Sequencing of Steroid Producing Bacteria with Illumina Technology

Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome

RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics

Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria

Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805

Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas

Selection of Biodegrading Phytosterol Strains

Stigmasterol Removal by an Aerobic Treatment System

Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione

Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates

Bioconversion of Phytosterols into Androstenedione by Mycobacterium

Scale-Up of Phytosterols Bioconversion into Androstenedione

Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331

Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)

Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants

Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols

Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus

Biotransformation of DHEA into 7α, 15α-diOH-DHEA

Stabilization of Enzymes by Using Thermophiles

Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media

Downstream Process Synthesis for Microbial Steroids

Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)

A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro. .

Other Format:

Printed edition:

ISBN:

9781493971831

Access Restriction:

Restricted for use by site license.