Lectin Methods and Protocols / edited by Jonathan M. Rhodes, Jeremy D. Milton.

Format:

Book

Contributor:

Rhodes, Jonathan M., editor.

Milton, Jeremy D., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 9.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 9

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (XVIII, 616 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. The book will immediately become the standard practical resource and guide to lectin techniques for today's biomedical researchers.

Contents:

Lectin Histochemistry and Cytochemistry

Light Microscopy

Lectin Histochemistry and Cytochemistry-Light Microscopy

Lectin-Gold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins

Use of Fluorochrome-Labeled Lectins in Light Microscopy

The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage

Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis

Electron Microscopy

Amplification of Lectin-Gold Histochemistry

Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines

Use of Lectins for Structural Analysis of Oligosaccharide Chains

Application of Sequential Smith Degradation to Lectin Blots

Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues

Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis

Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins

Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins

Use of Lectin for Detection of Agalactosyl IgG

Detection of Altered Glycosylation of ?-Fetoprotein Using Lectin-Affinity Electrophoresis

Use of Lectin-Affinity Electrophoresis for Quantification and Characterization of Glycoforms of ?-1 Acid Glycoprotein

ABO(H) Blood Group Expression on Circulating Glycoproteins

Use of Lectins in Quantification of Soluble Glycoproteins

Lectin/Antibody "Sandwich" ELISA for Quantification of Circulating Mucin as a Diagnostic Test for Pancreatic Cancer

Quantification of Intestinal Mucins

Lectins in Affinity Purification of Soluable Glycoproteins

Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin

Use of Lectins in Affinity Purification of HIV and SIV Envelope Glycoproteins

T-Cell Receptor Purification

Lectins in Flow Cytometry

Use of Monomeric, Monovalent Lectin Derivatives for Flow Cytometric Analysis of Cell Surface Glycoconjugates

Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry

Lectins as Tools for Cell Purification/Purging

Lectins as Tools for the Purification of Liver Endothelial Cells

The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation

Combined Lectin/Monoclonal Antibody Purging of Bone Marrow for Use in Conjunction with Autologous Bone Marrow Transplantation in the Treatment of Multiple Myeloma

Effects of Lectins on Mammalian Cells

Mechanisms and Assessment of Mitogenesis

Mitogenic Effects of Lectins on Epithelial Cells

Use of Lectins as Mitogens for Lymphocytes

Effect of Lectins on Uptake of Polyamines

Effects of Lectins on Cytoskeletal Organization in Mammalian Cells

Effect of Lectins on Protein Kinase Activity

Lectin-Induced Calcium Mobilization in Human Platelets

Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells

Cytotoxic Effects of Lectins

Application of the Lectin-Dependent Cell-Mediated Cytotoxicity Assay to Bronchoalveolar Lavage Fluid and Venous Blood Samples Collected from Canine Lung Allografts

Effects of Lectins in Organ Culture

The Effect of Lectins on Crypt Cell Proliferation in Organ Culture

Effects of Lectin Ingestion

Effects of Lectin Ingestion on Animal Growth and Internal Organs

Lectin Ingestion

Assessment of Lectin Inactivation by Heat and Digestion

Lectins in The Investigation of Neuronal Trafficking

Use of Lectins as Transganglionic Neuronal Tracers in the Study of Unmyelinated Primary Sensory Neurons

Use of Lectins in The Investigation of Pathogen-Host Interactions

Lectin Inhibition of Bacterial Adhesion to Animal Cells

Inhibition of HIV Infection by Lectin Binding to CD4

Inhibition of HIV Infection by Lectin Binding to gp120

Use of Lectins for Drug Delivery

Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis

The Use of Lectins for Liposome Targeting in Drug Delivery.

Other Format:

Printed edition:

ISBN:

9781592595938

Access Restriction:

Restricted for use by site license.