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Neural Stem Cells: Methods and Protocols / edited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 198.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 198
- Language:
- English
- Subjects (All):
- Medicine.
- Neurosciences.
- Neurobiology.
- Biomedicine.
- Local Subjects:
- Biomedicine.
- Neurosciences.
- Neurobiology.
- Physical Description:
- 1 online resource (XIV, 381 pages) : 196 illustrations, 43 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press : Imprint: Humana Press, 2002.
- System Details:
- text file PDF
- Summary:
- Over the last decade, advances in neural stem cell research have raised the hope that one day cellular therapy will not only replace missing neurons, but also replenish absent chemical signals, metabolites, enzymes, neurotransmitters, or other missing or defective components from the diseased or injured brain. In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics. Each is written by an investigator who has used the method extensively, and includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Comprehensive and easy-to-follow, Neural Stem Cells: Methods and Protocols provides a powerful synthesis of today's key in vitro and in vivo techniques to identify and characterize neural stem cells for research and for developing experimental therapeutics.
- Contents:
- Isolation and Culture of NSCs
- Neural Differentiation of Embryonic Stem Cells
- Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens
- Isolation of Stem and Precursor Cells from Fetal Tissue
- Olfactory Ensheathing Cells
- Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium
- Production of Immortalized Human Neural Crest Stem Cells
- Adult Rodent Spinal Cord Derived Neural Stem Cells
- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood
- Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers
- Characterization of NSCs in VITRO
- Analysis of Cell Generation in theTelencephalic Neuroepithelium
- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
- Assessing the Involvement of Telomerase in Stem Cell Biology
- Detection of Telomerase Activity in Neural Cells
- In Vitro Assays for Neural Stem Cell Differentiation
- Electron Microscopy and Lac-Z Labeling
- Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
- Fluorescence In Situ Hybridization
- RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells
- Differential Display
- Cell Labeling and Gene Misexpression by Electroporation
- Gene Therapy Using Neural Stem Cells
- Modeling Brain Pathologies Using Neural Stem Cells
- Utilization/Characterization of NSCs in Vivo
- Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model
- Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods
- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
- Immunocytochemical Analysis of Neuronal Differentiation
- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
- Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS
- Optimizing Stem Cell Grafting into the CNS
- Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos
- Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells
- Routes of Stem Cell Administration in the Adult Rodent.
- Other Format:
- Printed edition:
- ISBN:
- 9781592591862
- Access Restriction:
- Restricted for use by site license.
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