Epigenetic Methods in Neuroscience Research / edited by Nina Karpova.

Format:

Book

Contributor:

Karpova, Nina, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 105.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 105

Language:

English

Subjects (All):

Neurosciences.

Local Subjects:

Neurosciences.

Physical Description:

1 online resource (XII, 258 pages) : 43 illustrations, 37 illustrations in color.

Edition:

First edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3'UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory. Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.

Contents:

Epigenetics: From Basic Biology To Chromatin Modifying Drugs And New Potential Clinical Applications

Analysis Of Brain Epigenome: A Guide To Epigenetic Methods

Analysis Of DNA Methylation By Bisulfite Reaction In Neural Cells As An Example Of Orexin Neurons

Protocol For The Simultaneous Analysis Of Gene DNA Methylation And Mrna Expression Levels In The Rodent Brain

Simultaneous Quantification Of Global 5mc And 5hmc Levels In The Nervous System Using A HPLC/MS Method

Protocol For Methylated DNA Immunoprecipitation (Medip) Analysis

Cell Type-Specific DNA Methylation Analysis In Neurons And Glia

Immunohistochemical Detection Of Oxidized Forms Of 5-Methylcytosine In Embryonic And Adult Brain Tissue

Analysis Of Histone Modifications In Neural Cells As An Example Of Orexin Neurons

Site-Specific Delivery Of Epigenetic Modulating Drugs Into The Rat Brain

Mammalian Genome Plasticity: Expression Analysis Of Transposable Elements

Tools For Analyzing The Role Of Local Protein Synthesis In Synaptic Plasticity

In Situ Detection Of Neuron-Specific Micrornas In Frozen Brain Tissue Meister

Protocol For High-Throughput Mirna Profiling Of The Rat Brain

Metabolites As Clock Hands: Estimation Of Internal Body Time Using Blood Metabolomics.

Other Format:

Printed edition:

ISBN:

9781493927548

Access Restriction:

Restricted for use by site license.