Stress Response : Methods and Protocols / edited by John M. Walker, Stephen M. Keyse.

Format:

Book

Contributor:

Walker, John M., editor.

Keyse, Stephen M., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 99.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 99

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XVI, 488 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2000.

System Details:

text file PDF

Summary:

In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease.

Contents:

Detection and Assay of Stress-Induced Damage

Identifying and Counting Protein Modifications Triggered by Nitrosative Stress

Determination of Carbonyl Groups in Oxidized Proteins

Quantitation of 4-Hydroxynonenal Protein Adducts

Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate

The Measurement of Protein Degradation in Response to Oxidative Stress

The Activation of Signal Transduction by Cellular Stress

Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress

Detection and Activation of Stress-Responsive Tyrosine Kinases

Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells

Expression and Assay of Recombinant ATM

Detection and Purification of a Multiprotein Kinase Complex from Mammalian Cells

Methods to Assay Stress-Activated Protein Kinases

Monitoring the Activation of Stress-Activated Protein Kinases Using GAL4 Fusion Transactivators

Use of Kinase Inhibitors to Dissect Signaling Pathways

The Development and Use of Phospho-Specific Antibodies to Study Protein Phosphorylation

Peptide Assay of Protein Kinases and Use of Variant Peptides to Determine Recognition Motifs

The Analysis of Stress-Induced Gene Expression

Assaying NF-KB and AP-1 DNA-Binding and Transcriptional Activity

Analysis of the Mammalian Heat-Shock Response

Approaches to Define the Involvement of Reactive Oxygen Species and Iron in Ultraviolet-A Inducible Gene Expression

The Human Immunodeficiency Virus LTR-Promoter Region as a Reporter of Stress-Induced Gene Expression

Sage: The Serial Analysis of Gene Expression

Analysis of Differential Gene Expression Using the SABRE Enrichment Protocol

UVB-Regulated Gene Expression in Human Keratinocytes

Analysis of Stress Protein Function

Heme Oxygenase Activity Current Methods and Applications

Analysis of Molecular Chaperone Activities Using In Vitro and In Vivo Approaches

Analysis of Chaperone Properties of Small Hsp'

Analysis of Small Hsp Phosphorylation

Analysis of Multisite Phosphorylation of the p53 Tumor-Suppressor Protein byTryptic Phosphopeptide Mappin

Development of Physiological Models to Study Stress Protein Responses.

Other Format:

Printed edition:

ISBN:

9781592590544

Access Restriction:

Restricted for use by site license.