Mitochondrial DNA : Methods and Protocols / edited by Matthew McKenzie.

Format:

Book

Contributor:

McKenzie, Matthew, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1351.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1351

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XI, 234 pages) : 43 illustrations, 23 illustrations in color.

Edition:

Third edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number, the latest technologies for modifying the mitochondrial genome, and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.

Contents:

Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing

Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g

Quantification Of DNA Damage By Real-Time Qpcr

Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule PCR

A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity

Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis

Tracking Mitochondrial DNA In Situ

Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis

Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria

Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA

Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasm

Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice

Import Of Fluorescent RNA Into Mitochondria Of Living Cells

Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase

Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster

Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein

Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome.

Other Format:

Printed edition:

ISBN:

9781493930401

Access Restriction:

Restricted for use by site license.