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Plant Epigenetics : Methods and Protocols / edited by Igor Kovalchuk, Franz J. Zemp.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 631.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 631
- Language:
- English
- Subjects (All):
- Life sciences.
- Gene expression.
- Biochemistry.
- Botany.
- Plant genetics.
- Life Sciences.
- Plant Genetics & Genomics.
- Biochemistry, general.
- Plant Sciences.
- Gene Expression.
- Local Subjects:
- Life Sciences.
- Plant Genetics & Genomics.
- Biochemistry, general.
- Plant Sciences.
- Gene Expression.
- Physical Description:
- 1 online resource (X, 273 pages) : 51 illustrations, 12 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2010.
- System Details:
- text file PDF
- Summary:
- The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and innovative, Plant Epigenetics: Methods and Protocols is an invaluable manual designed to help researchers uncover the undiscovered and unexplained phenomena in plant biology.
- Contents:
- Analysis of DNA Methylation in Plants by Bisulfite Sequencing
- Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE
- Analysis of Locus-Specific Changes in Methylation Patterns Using a COBRA (Combined Bisulfite Restriction Analysis) Assay
- Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay
- In Situ Analysis of DNA Methylation in Plants
- Analysis of Mutation/Rearrangement Frequencies and Methylation Patterns at a Given DNA Locus Using Restriction Fragment Length Polymorphism
- Isoschizomers and Amplified Fragment Length Polymorphism for the Detection of Specific Cytosine Methylation Changes
- Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays
- Northern Blotting Techniques for Small RNAs
- qRT-PCR of Small RNAs
- Cloning New Small RNA Sequences
- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part A: ChIP-chip Molecular Methods
- Genome-Wide Mapping of Protein-DNA Interaction by Chromatin Immunoprecipitation and DNA Microarray Hybridization (ChIP-chip). Part B: ChIP-chip Data Analysis
- Metaanalysis of ChIP-chip Data
- Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
- cDNA Libraries for Virus-Induced Gene Silencing
- Detection and Quantification of DNA Strand Breaks Using the ROPS (Random Oligonucleotide Primed Synthesis) Assay
- Reporter Gene-Based Recombination Lines for Studies of Genome Stability
- Plant Transgenesis.
- Other Format:
- Printed edition:
- ISBN:
- 9781607616467
- Access Restriction:
- Restricted for use by site license.
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