Plant Immunity : Methods and Protocols / edited by John M. McDowell.

Format:

Book

Contributor:

McDowell, John M., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 712.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 712

Language:

English

Subjects (All):

Life sciences.

Botany.

Plant diseases.

Life Sciences.

Plant Sciences.

Plant Pathology.

Local Subjects:

Life Sciences.

Plant Sciences.

Plant Pathology.

Physical Description:

1 online resource (XV, 280 pages).

Edition:

1.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Contents:

Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression

Fragment Complementation and Co-Immunoprecipitation Assays for Understanding R Protein Structure and Function

Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

Biochemical Purification of Native Immune Protein Complexes

Chromatin Immunoprecipitation (ChIP) to Identify Global Targets of WRKY Transcription Factor Family Members Involved in Plant Immunity

Dose Response to and Systemic Movement of Dexamethasone in the GVG Inducible Transgene System in Arabidopsis

Quantifying Alternatively Spliced mRNA via Capillary Electrophoresis

Constructing Haustorium-Specific cDNA Libraries from Rust Fungi

Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes

Construction of Pseudomonas syringae pv. tomato DC3000 Mutant and Polymutant Strains

A Straightforward Protocol for Electro-Transformation of Phytophthora capsici Zoospores

Propagation, Storage, and Assays with Hyaloperonospora arabidopsidis, a Model Oomycete Pathogen of Arabidopsis

Assaying Effector Function In planta Using Double-Barreled Particle Bombardment

Assays for Effector-Mediated Suppression of Programmed Cell Death in Yeast

Purification of Effector-Target Protein Complexes via Transient Expression in N. benthamiana

Imaging Fluorescently-Tagged Phytophthora Effector Proteins Inside Infected Plant Tissue

Immunolocalization of Pathogen Effectors

Laser Capture Microdissection of Nematode Feeding Cells

Laser Microdissection of Plant-Fungus Interaction Sites and Isolation of RNA for Downstream Expression Profiling

Global Expression Profiling of RNA from Laser Microdissected Cells at Fungal-Plant Interaction Sites

Visualizing Cellular Dynamics in Plant-Microbe Interactions Using Fluorescent-Tagged Proteins.

Other Format:

Printed edition:

ISBN:

9781617379987

Access Restriction:

Restricted for use by site license.