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Transgenesis Techniques : Principles and Protocols / edited by Alan R. Clarke.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 180.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, 1064-3745 ; 180
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Life Sciences.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Physical Description:
- 1 online resource (X, 351 pages).
- Edition:
- Second Edition.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Springer New York, 2002.
- System Details:
- text file PDF
- Summary:
- The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.
- Contents:
- Topical Reviews in Transgenesis
- Biomedical and Agricultural Applications of Animal Transgenesis
- Transgenesis in Invertebrate and Lower Vertebrate Species
- Gene Transfer in Drosophila
- Transgenesis in the Mouse: Oocyte Injection
- Oocyte Injection in the Mouse
- Alternative Routes to the Germline
- Adenoviral Infection
- Retroviral Infection
- Transgenesis in the Mouse: The ES Cell Route
- In Vitro Isolation of Murine Embryonic Stem Cells
- Production of Chimeras Derived from Murine Embryonic Stem Cells
- Gene-Targeting Strategies
- Cre/loxP Recombination System and Gene Targeting
- Cryopreservation of Mouse Lines
- Cryopreservation of Transgenic Mouse Lines
- Ovarian Tissue Transplantation and Cryopreservation
- Transgenesis in the Rat
- Transgenesis in Domestic Species
- Generation of Transgenic Livestock by Pronuclear Injection
- Transgenic Sheep from Cultured Cells
- Characterization and Analysis of Transgenic Strains
- Analysis of Transgenic Mice.
- Other Format:
- Printed edition:
- ISBN:
- 9781592591787
- Access Restriction:
- Restricted for use by site license.
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