Autoimmunity : Methods and Protocols / edited by Andras Perl.

Format:

Book

Contributor:

Perl, Andras, editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 102.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 102

Language:

English

Subjects (All):

Medicine.

Immunology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Physical Description:

1 online resource (XIV, 434 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2004.

System Details:

text file PDF

Summary:

Autoimmunity-which affects up to 10% of the world's population-can damage nearly every tissue or cell type of the human body with greatly debilitating or fatal results. In Autoimmunity: Methods and Protocols, expert medical researchers describe in step-by-step detail molecular techniques and in vitro/in vivo model systems for discovering the causes of autoimmunity, as well as how best it may be regulated. The methods to assess the immunological and biochemical pathways relevant for pathogenesis include identifying susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor, signal processing via protein kinases, identification and enumeration of antigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Additional protocols serve to establish the presence of and assess inflammatory arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, multiple sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo. These methods can be used to assess the genetic, immunological, and biochemical parameters underlying spontaneous or exogenous antigen-induced diseases. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and easy to follow, Autoimmunity: Methods and Protocols offers today's investigators a cutting-edge collection of readily reproducible methods for establishing and interrogating human and animal models of autoimmune diseases.

Contents:

Pathogenesis and Spectrum of Autoimmunity

Pathogenesis and Spectrum of Autoimmunity

Models of Pathogenesis in Human Autoimmune Disorders

Mapping the Systemic Lupus Erythematosus Susceptibility Genes

T-Cell Signaling Abnormalities in Human Systemic Lupus Erythematosus

TCR ?-Chain Abnormalities in Human Systemic Lupus Erythematosus

Protein Kinase A and Signal Transduction in T Lymphocytes

Apoptosis and Mitochondrial Dysfunction in Lymphocytes of Patients With Systemic Lupus Erythematosus

Methods for Inducing Apoptosis

Measurement of Cytokines in Autoimmune Disease

Evaluation of Autoimmunity to Transaldolase in Multiple Sclerosis

Animal Models of Autoimmunity

Animal Models for Autoimmune Myocarditis and Autoimmune Thyroiditis

Animal Models of Insulin-Dependent Diabetes

Generation, Maintenance, and Adoptive Transfer of Diabetogenic T-Cell Lines/Clones From the Nonobese Diabetic Mouse

Experimental Use of Murine Lupus Models

The Anti-DNA Knock-In Model of Systemic Autoimmunity Induced by the Chronic Graft-vs-Host Reaction

Murine Models of Lupus Induced by Hypomethylated T Cells

The Mouse Model of Collagen-Induced Arthritis

Proteoglycan Aggrecan-Induced Arthritis

Mouse Models of Multiple Sclerosis

Experimental Autoimmune Encephalomyelitis

Animal Models of Scleroderma

Rodent Models of Experimental Autoimmune Uveitis

Autoimmune Depigmentation Following Sensitization to Melanoma Antigens.

Other Format:

Printed edition:

ISBN:

9781592598052

Access Restriction:

Restricted for use by site license.